Cardiomyocyte H9c2 cells present a valuable alternative to fish lethal testing for azoxystrobin

Environmental Pollution
Elsa T RodriguesTeresa L Serafim

Abstract

The present study aims at identifying, among six mammalian and fish cell lines, a sensitive cell line whose in vitro median inhibitory concentration (IC50) better matches the in vivo short-term Sparus aurata median lethal concentration (LC50). IC50s and LC50 were assessed after exposure to the widely used fungicide azoxystrobin (AZX). Statistical results were relevant for most cell lines after 48 h of AZX exposure, being H9c2 the most sensitive cells, as well as the ones which provided the best prediction of fish toxicity, with a LC50,96h/IC50,48h = 0.581. H9c2 cell proliferation upon 72 h of AZX exposure revealed a LC50,96h/IC50,72h = 0.998. Therefore, identical absolute sensitivities were attained for both in vitro and in vivo assays. To conclude, the H9c2 cell-based assay is reliable and represents a suitable ethical alternative to conventional fish assays for AZX, and could be used to get valuable insights into the toxic effects of other pesticides.

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Jan 20, 1998·Journal of Immunological Methods·K T PapazisisA H Kortsaris
Mar 10, 2001·Biochemical and Biophysical Research Communications·A A Starkov, G Fiskum
Jul 31, 2002·Pest Management Science·Dave W BartlettBob Parr-Dobrzanski
Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·S Ní ShúilleabháinM Davoren
Dec 16, 2004·Environmental Science & Technology·Steven P BradburyCornelis J Van Leeuwen
Apr 5, 2007·Nature Protocols·Vanicha Vichai, Kanyawim Kirtikara
Nov 13, 2008·Cytotechnology·Cátia L MarquesVincent Laizé
Aug 5, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nynke I KramerKristin Schirmer
Jan 15, 2013·Environment International·Elsa Teresa RodriguesMiguel Ângelo Pardal
May 28, 2013·Archives of Toxicology·Wiebke PrutnerPablo Steinberg

❮ Previous
Next ❯

Citations

Apr 25, 2016·Bulletin of Environmental Contamination and Toxicology·P DvorakM Tomko
Dec 29, 2019·Environmental Science and Pollution Research International·Elsa T RodriguesVilma A Sardão
Mar 11, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Bruno M MoukettePaulo J Oliveira
Feb 7, 2018·Ecotoxicology and Environmental Safety·Siyu WuDefu He
Mar 22, 2020·Ecotoxicology and Environmental Safety·Raquel A F NevesElsa T Rodrigues

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Proceedings of the Western Pharmacology Society
C J Hilado, A Furst
Toxicology in Vitro : an International Journal Published in Association with BIBRA
A YangF A Barile
© 2021 Meta ULC. All rights reserved