Cardiomyocytic apoptosis limited by bradykinin via restoration of nitric oxide after cardioplegic arrest

The Journal of Surgical Research
Chi-Hsiao YehShu-Wen Fang

Abstract

Our previous studies revealed that cardioplegia-induced cardiac arrest under cardiopulmonary bypass (CPB) decreased cardiomyocytic nitric oxide and increased apoptosis. We hypothesized that pretreatment with bradykinin (BK) would improve the profile of anti-apoptotic proteins and inhibit cardiomyocytic apoptosis. New Zealand white rabbits received total CPB. Rabbits were weaned from CPB and reperfused for 4 h. Blood was sampled at various time points. Bradykinin and/or nitric oxide synthase (NOS) inhibitors or BK-receptor antagonists were infused systemically 30 min before beginning of CPB, and continued throughout the procedure. The ascending aorta was cross-clamped for 60 min while cold crystalloid cardioplegic solution was intermittently infused into the aortic root. The hearts were harvested and studied for evidence of apoptosis and ischemia/reperfusion induced inflammation-related cytokine production by cardiomyocytes. Our results revealed that bradykinin supplementation during cardioplegia could prevent I/R-induced inflammatory and apoptotic effects, which could be reversed with a NOS inhibitor. BK antagonists and NOS inhibitors worsened the inflammatory and apoptotic responses of cardiomyocytes, which could be reversed w...Continue Reading

References

Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Catherine CommunalRoger J Hajjar
Jun 28, 2003·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Uwe M FischerUwe Mehlhorn
Dec 24, 2005·Circulation Research·Christophe DepreStephen F Vatner
Jan 29, 2008·The Journal of Biological Chemistry·Karin KaiserovaAruni Bhatnagar

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Citations

Aug 10, 2010·The Journal of Surgical Research·Tim Lahm
Jun 5, 2016·American Journal of Cardiovascular Drugs : Drugs, Devices, and Other Interventions·Stefano Taddei, L Bortolotto
Jul 11, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Miao HeHong Zhang

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