PMID: 20629327Jul 16, 2010Paper

Cardioprotective effects of St. Thomas solution containing PolyHb on isolated rat heart during cold preservation

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Pu ZhangJin Liu

Abstract

To investigate the cardioprotective effects of St. Thomas solution containing PolyHb on ischemia-reperfusion injury to isolated rat heart after eight-hour cold storage. Sixty male inbred SD rats were randomly divided into 3 groups (n = 20 in each group): sham group, St. Thomas solution (STS) group and STS with PolyHb group. The Langendoff isolated rat heart model was used. The hearts were stored in 4 degrees C St. Thomas solution with or without PolyHb for 8 h followed by 120 min of reperfusion. The recovery of cardiac function was observed. Myocardial enzyme leakage and infarct size were measured after reperfusion. In comparison with STS group, St. Thomas solution containing PolyHb improved the recovery of LVDP (P < 0.001) and +/- dp/dt (P < 0.001), decreased the release of CK and LDH (P < 0.001), reduced the percentage of the infarct size (P < 0.001) of the heart during reperfusion as well. Adding PolyHb to St. Thomas solution can protect myocardium from ischemia-reperfusion injury after eight-hour cold preservation.