Cardioprotective role of azafrin in against myocardial injury in rats via activation of the Nrf2-ARE pathway

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Shuyu YangQinglin Li

Abstract

The root of Centranthera grandiflora Benth. is a Chinese ethnodrug known as Ye-Can-Dou-Gen (YCDG) and has been widely used to treat cardiovascular diseases in Hmong, Yunnan Province, China; however, its mechanisms of action were largely unknown. Azafrin, a natural carotenoid, is one of the most abundant active ingredients in YCDG. We aimed to explore the cardioprotective effects of azafrin on myocardial infarction (MI) and myocardial ischaemia-reperfusion (MIR) injury and to clarify its underlying myocardial protective mechanisms. The in vitro cardioprotective effects of azafrin against oxidative stress injury in H9c2 cells were assessed by the ferric reducing antioxidant power (FRAP) assay and a viability assay in the presence of H2O2-induced oxidative insults, whereas the in vivo effects were assessed with the echocardiographic and histopathological evaluation together with 2,3,5-triphenyltetrazolium chloride (TTC) and serum biochemical analyses in vivo. Then, we used the luciferase reporter gene assay to measure Nrf2 transcriptional activity in HEK 293 cells and analysed the expression levels of enzymes downstream of Nrf2-ARE signalling by q-PCR and western blotting. Azafrin showed higher FRAP values than FeSO4 and exhibited...Continue Reading

Citations

Dec 5, 2019·International Journal of Molecular Sciences·Xiaodong ZhangWensheng Qin
Nov 27, 2019·Journal of Bioenergetics and Biomembranes·Liangtong LiFulin Liu
Mar 29, 2019·Oxidative Medicine and Cellular Longevity·Xinyu YangHongcai Shang
May 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michaela KosutovaMartina Cebova
Oct 12, 2020·Pharmacology & Therapeutics·Chunhui ZhaoLuqi Huang
May 9, 2021·The Journal of Pharmacy and Pharmacology·Fan LiuZheng Ji
Oct 5, 2021·Journal of Asian Natural Products Research·Xin-Xin LiangWei-Lie Xiao
Nov 7, 2020·Current Vascular Pharmacology·Rasool TavakoliAmirhossein Sahebkar

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