Cardosin A endocytosis mediated by integrin leads to lysosome leakage and apoptosis of epithelial cells

Proteins
Hao WuJordan Tang

Abstract

Cardosin A is an aspartic protease present in large amount in the pistils of cardoon flowers. This protease is known to contain an -Arg-Gly-Asp- (RGD) motif located on the molecular surface. In this study, we found that isolated recombinant cardosin A attached to human epithelial cells A549, mediated by the binding of its RGD motif to cell surface integrins. The cell bound cardosin A was internalized to endosomes and lysosomes and triggered the permeability of lysosomal membrane leading to apoptosis of the epithelial cells. These events are identical to those observed for three RGD-containing aspartic proteases, Saps 4-6, secreted by Candida albicans. Such a process, which has been called the Trojan Horse mechanism, is believed to benefit the invasion of C. albican into the epithelium of the host. The location of the RGD motifs of cardosin A and Saps 4-6 are on the opposite ends of the homologous three-dimensional structures, suggesting that the Trojan Horse mechanism is insensitive to the RGD position. Current finding also suggests that cardosin A may have a defensive function against the ingestion of cardoon flowers by human, insects, and other herbivores.

References

May 2, 2002·Genome Biology·Ben M DunnAlexander Wlodawer
Oct 4, 2002·Developmental Cell·Christian Bökel, Nicholas H Brown
Nov 7, 2002·Current Biology : CB·Urs ErikssonJosef M Penninger
Sep 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Julian R NaglikBernhard Hube
Nov 1, 2003·The Lancet Infectious Diseases·Philippe EggimannDidier Pittet
May 22, 2004·European Journal of Biochemistry·Isaura Simões, Carlos Faro
Feb 6, 2007·Free Radical Biology & Medicine·Paschalis-Thomas DouliasCharalampos Angelidis
Nov 30, 2007·Free Radical Biology & Medicine·Peter G ArthurGary P Jeffrey
Jul 16, 2008·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Arun K GhoshJordan Tang
Feb 23, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hao WuJordan Tang
Mar 11, 2015·Dermatitis : Contact, Atopic, Occupational, Drug·Alexandra PriceDavid E Cohen
Oct 11, 2017·Tüberküloz ve toraks·Özlem Kar KurtMevlüt Kurt
Nov 1, 2017·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·D LetnerJ Garber

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