Cardosins improve neuronal regeneration after cell disruption: a comparative expression study

Cell Biology and Toxicology
Ana S DuarteMarlene T Barros

Abstract

The establishment of primary cell cultures is invaluable for studying cell and molecular biological questions. Although primary cell cultures more closely resemble and function like in the native environment, during the culture establishment the cells undergo several changes including the damage sustained during their removal from original tissue. The resultant cells have to rebalance the expression of their processing molecules to ascertain matrix signalling that ensure cell adaptation and consequent proliferation. Hence, we used cardosin, a novel plant enzyme for tissue disaggregation, for isolating and culturing neuronal cells from embryonic rats. The present investigation reports the molecular events, mainly related with matrix metalloproteinases (MMPs)/tissue inhibitor of metalloproteinase (TIMPs) expression, which could substantiate the superior neurite outgrowth and dendritic extension previously described. It was observed that 24 h after primary culture establishment, MMP-2 and MMP-9 messenger RNA (mRNA) are significantly upregulated, while the expression of TIMP-1 and TIMP-2 is unaltered. Regarding the role of laminin in neuronal pathfinding, it was found that the use of anti-laminin antibody and arginine-glycine-aspar...Continue Reading

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Citations

May 25, 2013·Phytochemistry·Sandra Vairo CavalliNora Priolo
Apr 24, 2014·Critical Reviews in Microbiology·Ana Sofia DuarteAna Cristina Esteves
Aug 19, 2016·Frontiers in Plant Science·Carina FélixAna C Esteves
Jan 24, 2018·International Journal of Molecular Sciences·Carina FélixAna C Esteves
Dec 3, 2011·Molecular Biology and Evolution·Romain Derelle, B Franz Lang

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