Caregiver maltreatment causes altered neuronal DNA methylation in female rodents

Development and Psychopathology
Jennifer Blaze, Tania L Roth

Abstract

Negative experiences with a caregiver during infancy can result in long-term changes in brain function and behavior, but underlying mechanisms are not well understood. It is our central hypothesis that brain and behavior changes are conferred by early childhood adversity through epigenetic changes involving DNA methylation. Using a rodent model of early-life caregiver maltreatment (involving exposure to an adverse caregiving environment for postnatal days 1-7), we have previously demonstrated abnormal methylation of DNA associated with the brain-derived neurotrophic factor (Bdnf) gene in the medial prefrontal cortex (mPFC) of adult rats. The aim of the current study was to characterize Bdnf DNA methylation in specific cell populations within the mPFC. In the prefrontal cortex, there is approximately twice as many neurons as glia, and studies have recently shown differential and distinctive DNA methylation patterns in neurons versus nonneurons. Here, we extracted nuclei from the mPFC of adult animals that had experienced maltreatment and used fluorescence-activated cell sorting to isolate cell types before performing bisulfite sequencing to estimate methylation of cytosine-guanine sites. Our data indicate that early-life stress ...Continue Reading

References

Mar 24, 2017·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Jennifer BlazeTania L Roth
Dec 5, 2017·Policy Insights From the Behavioral and Brain Sciences·Tania L Roth
Feb 28, 2019·Developmental Psychobiology·Samantha M KellerTania L Roth
Apr 13, 2017·Development and Psychopathology·Glenn I Roisman, Dante Cicchetti
Mar 16, 2019·Frontiers in Neuroscience·Laura R CortesNancy G Forger
Oct 23, 2019·Translational Psychiatry·Alex FerrerVirginia Soria
Oct 13, 2018·Developmental Psychobiology·Amanda C KentnerSusanne Brummelte
Sep 27, 2019·Frontiers in Behavioral Neuroscience·Rodrigo OrsoRodrigo Grassi-Oliveira
Jun 8, 2018·Frontiers in Molecular Neuroscience·Gonca Bayraktar, Michael R Kreutz

Citations

Aug 1, 1979·Journal of Comparative and Physiological Psychology·C L Moore, G A Morelli
May 1, 1995·Journal of the American Academy of Child and Adolescent Psychiatry·D Cicchetti, Sheree L Toth
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·C CaldjiMichael J Meaney
Aug 5, 2000·JAMA : the Journal of the American Medical Association·Christine HeimCharles B Nemeroff
Jan 4, 2001·Biological Psychiatry·C CaldjiMichael J Meaney
Jul 5, 2001·Physiology & Behavior·Enrico Alleva, D Santucci
Aug 2, 2001·Journal of Biochemistry·K Nakajima, S Kohsaka
Aug 9, 2001·Journal of Neuroscience Research·K NakajimaT Kurihara
Nov 9, 2001·Child Development·Mary DozierB Bates
Jul 3, 2002·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ann M RasmussonRonald Duman
Nov 27, 2002·The American Journal of Psychiatry·Meena VythilingamJ Douglas Bremner
May 7, 2003·Neuroscience and Biobehavioral Reviews·Martin H TeicherDennis M Kim
Jun 29, 2004·Nature Neuroscience·Ian C G WeaverMichael J Meaney
Jul 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Lisa M MonteggiaEric J Nestler
Jul 27, 2004·Nature Neuroscience·Christian MirescuElizabeth Gould
Dec 21, 2004·Journal of Molecular Histology·Catherine P RileyCharles R Buck
May 17, 2005·American Journal of Preventive Medicine·Shanta R DubeWayne H Giles
Apr 18, 2006·Biological Psychiatry·Ronald A CohenLeanne M Williams
Dec 7, 2006·Journal of Neuroscience Research·Tamara AidTõnis Timmusk
Dec 13, 2006·Development and Psychopathology·Dante CicchettiSheree L Toth
Feb 20, 2007·Brain Research·Michail LeventopoulosJolanta Opacka-Juffry
Aug 25, 2007·Annual Review of Clinical Psychology·Dante Cicchetti, Sheree L Toth
Aug 30, 2007·Nature Neuroscience·Keri MartinowichBai Lu
Jun 10, 2008·Epigenetics : Official Journal of the DNA Methylation Society·Tim F OberlanderAngela M Devlin
Jul 10, 2008·The International Journal of Neuropsychopharmacology·Xuefei WuJau-Shyong Hong
Sep 5, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Bridget R Mueller, Tracy L Bale
Sep 27, 2008·Neuroscience and Biobehavioral Reviews·Francesca CirulliStephen J Suomi

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Guanine
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