Nov 17, 2016

Cargo Transport by Two Coupled Myosin Va Motors on Actin Filaments and Bundles

Biophysical Journal
M Yusuf AliDavid M Warshaw

Abstract

Myosin Va (myoVa) is a processive, actin-based molecular motor essential for intracellular cargo transport. When a cargo is transported by an ensemble of myoVa motors, each motor faces significant physical barriers and directional challenges created by the complex actin cytoskeleton, a network of actin filaments and actin bundles. The principles that govern the interaction of multiple motors attached to the same cargo are still poorly understood. To understand the mechanical interactions between multiple motors, we developed a simple in vitro model in which two individual myoVa motors labeled with different-colored Qdots are linked via a third Qdot that acts as a cargo. The velocity of this two-motor complex was reduced by 27% as compared to a single motor, whereas run length was increased by only 37%, much less than expected from multimotor transport models. Therefore, at low ATP, which allowed us to identify individual motor steps, we investigated the intermotor dynamics within the two-motor complex. The randomness of stepping leads to a buildup of tension in the linkage between motors-which in turn slows down the leading motor-and increases the frequency of backward steps and the detachment rate. We establish a direct relati...Continue Reading

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Mentioned in this Paper

Melanocyte
Conjugation
Alb
Gait, Drop Foot
Cell Motility
Myo5a protein, mouse
Actins
Complex (molecular entity)
Protoplasm
FSCN1

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