Carica papaya latex is a rich source of a class II chitinase

Phytochemistry
Mohamed AzarkanY Looze

Abstract

A class II chitinase is present in the latex of the tropical species Carica papaya. The enzyme may be readily purified by using a combination of hydrophobic interaction- and cation-exchange chromatography. This enzyme preparation is homogeneous with respect to the three physico-chemical criteria of charge, M(r) (28,000) and hydrophobicity. It is also completely free of any proteolytic and bacteriolytic activities. The enzyme was classified as a class II chitinase on the basis of its N-terminal amino acid sequence up to the 30th residue. In agreement with this classification, the enzyme preparation hydrolyses chitinase substrates only very slowly and several free thiol functions are present in the polypeptide chain. These free thiol functions are buried, and to be available for titration with 2,2'-dipyridyldisulphide, the enzyme must be denatured. Unfolding of papaya chitinase requires particularly drastic conditions, not less than 4 M guanidinium hydrochloride at 25 degrees and pH 6.8.

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Citations

Mar 9, 2004·Phytochemistry·Mohamed AzarkanDanielle Baeyens-Volant
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohamed AzarkanYvan Looze
Oct 24, 2008·Phytopathology·Kenneth G MooreGary A Payne
Oct 5, 2010·PPAR Research·Yuji NaitoToshikazu Yoshikawa
Oct 29, 2010·PPAR Research·Alexandra RogueAndré Guillouzo
Dec 29, 2009·PPAR Research·Christine Linard, Maâmar Souidi
Sep 26, 2012·PPAR Research·Neerupma SilswalJon Andresen
Sep 12, 2012·PPAR Research·Francesca AgriestiClaudia Piccoli
Jun 20, 2013·Mediators of Inflammation·Francisco A MonsalveRodrigo Moore-Carrasco
Aug 25, 2012·PPAR Research·Anthony J Apostoli, Christopher J B Nicol
Jul 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jefferson Soares de OliveiraMárcio Viana Ramos
Jan 21, 2004·The Plant Journal : for Cell and Molecular Biology·Kotaro KonnoKatsuyuki Kohno
Apr 4, 2006·International Journal of Biological Macromolecules·Mohamed AzarkanDanielle Baeyens-Volant
Oct 21, 2016·Plant Physiology and Biochemistry : PPB·Cleverson D T FreitasMárcio V Ramos
Sep 25, 2007·Plant Physiology and Biochemistry : PPB·Cleverson Diniz T FreitasMárcio Viana Ramos
Feb 1, 2007·Journal of Agricultural and Food Chemistry·Yu-Ting ChenJei-Fu Shaw

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