Carotenoid accumulation and carotenogenic gene expression during fruit development in novel interspecific inbred squash lines and their parents

Journal of Agricultural and Food Chemistry
Korakot NakkanongMingfang Zhang

Abstract

Carotenoid levels and composition during squash fruit development were compared in Cucurbita moschata , Cucurbita maxima , and two lines of their interspecific inbred lines, namely, Maxchata1 and Maxchata2. Eight genes associated with carotenoid biosynthesis were analyzed by quantitative RT-PCR. The two squash species and their interspecific inbred lines exhibited different qualitative and quantitative carotenoid profiles and regulatory mechanisms. C. moschata had the lowest total carotenoid content and mainly accumulated α-carotene and β-carotene, as expected in a fruit with pale-orange flesh. Low carotenoid content in this species was probably due to the comparatively low expression of all genes investigated, especially PSY1 gene, compared to the other squashes. The predominant carotenoids in C. maxima were violaxanthin and lutein, which produced a corresponding yellow flesh color in mature fruit. The relationship between the expression of the CHYB and ZEP genes may result in almost equal concentrations of violaxanthin and lutein in C. maxima at fruit ripening. In contrast, their interspecific inbred lines principally accumulated lutein and β-carotene, leading to orange flesh color. The PSY1 gene exhibited higher expression l...Continue Reading

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Citations

Jun 19, 2013·Journal of Agricultural and Food Chemistry·Ángeles ObreroBelén Román
Oct 28, 2015·Horticulture Research·Hui YuanLi Li
Aug 11, 2016·Food Chemistry·Shifeng CaoZhenfeng Yang
Sep 22, 2018·Molecular Breeding : New Strategies in Plant Improvement·Karolina KaźmińskaGrzegorz Bartoszewski

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