Carotenoid biosynthesis and sequestration in red chilli pepper fruit and its impact on colour intensity traits

Journal of Experimental Botany
Harriet M BerryPaul D Fraser

Abstract

The exploitation of diverse natural variation has been a key progenitor of crop breeding over the last decade. However, commercial practice is now turning to the use of accessions with less extreme phenotypes as genetic donors. In the present study, the carotenoid formation in a red-fruited discovery panel of Capsicum annuum (chilli pepper) has been characterized. The data indicated that colour intensity correlated with the amount of capsanthin and its esters, along with transcript levels of the 1-deoxy-d-xylulose 5-phosphate synthase (DXS) and phytoene synthase-1 (PSY-1) genes. Quantification of carotenoids through development and ripening suggested the presence of separate biosynthesis and accumulation phases. Subplastid fractionation demonstrated the differential sequestration of pigments in high- and low-intensity lines and revealed the PSY protein to be most active in the membrane fractions when abundance was highest in the fibril fractions. Carotenoid accumulation was associated with the esterification of xanthophylls, expression of a putative carotenoid acyl transferase, and increased fibril content within the plastid. Interrogation of TEM images and carotenoid analysis of subplastid fractions suggest that the plastoglob...Continue Reading

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Citations

Dec 5, 2019·International Journal of Molecular Sciences·Xiaodong ZhangWensheng Qin
Feb 24, 2020·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Soo Bin LeeJe Min Lee
Sep 15, 2020·Functional Plant Biology : FPB·Xiaochun WeiXiaowei Zhang
Nov 30, 2019·Plant Science : an International Journal of Experimental Plant Biology·Tianhu Sun, Li Li
Dec 3, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Guadalupe Villa-Rivera, Neftalí Ochoa-Alejo
Feb 19, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Xiongjie ZhengSalim Al-Babili
Apr 30, 2020·Progress in Lipid Research·Peter K LundquistRoberto Espinoza-Corral
Mar 20, 2021·Vavilovskiĭ Zhurnal Genetiki I Selekt︠s︡ii·E A DyachenkoE Z Kochieva
Sep 11, 2019·Plant Physiology and Biochemistry : PPB·Dario PianoDomenica Farci
Mar 31, 2021·The Plant Journal : for Cell and Molecular Biology·Roberto Espinoza-CorralPeter K Lundquist
Oct 28, 2021·Journal of the Science of Food and Agriculture·María Carmen PiñeroFrancisco M Del Amor

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Methods Mentioned

BETA
genetic drag
PCR
transgenic

Software Mentioned

ImageJ
BLAST

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