Carotenoid-chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29

Photosynthesis Research
Christoph-Peter HolleboomPeter J Walla

Abstract

It is known that aggregation of isolated light-harvesting complex II (LHCII) in solution results in high fluorescence quenching, reduced chlorophyll fluorescence lifetime, and increased electronic coupling of carotenoid (Car) S1 and chlorophyll (Chl) Qy states, as determined by two-photon studies. It has been suggested that this behavior of aggregated LHCII mimics aspects of non-photochemical quenching processes of higher plants and algae. However, several studies proposed that the minor photosystem II proteins CP24 and CP29 also play a significant role in regulation of photosynthesis. Therefore, we use a simple protocol that allows gradual aggregation also of CP24 and CP29. Similarly, as observed for LHCII, aggregation of CP24 and CP29 also leads to increasing fluorescence quenching and increasing electronic Car S1-Chl Qy coupling. Furthermore, a direct comparison of the three proteins revealed a significant higher electronic coupling in the two minor proteins already in the absence of any aggregation. These differences become even more prominent upon aggregation. A red-shift of the Qy absorption band known from LHCII aggregation was also observed for CP29 but not for CP24. We discuss possible implications of these results for...Continue Reading

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Citations

Sep 26, 2015·Journal of Photochemistry and Photobiology. B, Biology·Christopher D P Duffy, Alexander V Ruban
Mar 24, 2021·The Journal of Physical Chemistry Letters·Václav ŠebelíkTomáš Polívka
Apr 21, 2021·Physical Chemistry Chemical Physics : PCCP·Daniel A GacekPeter J Walla
Oct 25, 2017·The Journal of Physical Chemistry. B·Daniel A GacekPeter Jomo Walla
May 2, 2019·The Journal of Physical Chemistry Letters·Gabriel de la Cruz ValbuenaGiulio Cerullo

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