PMID: 6110720Nov 1, 1980Paper

Carrageenan-induced activation of human platelets is independent of phospholipase A2 and of formation of thromboxanes

The Journal of Pharmacy and Pharmacology
B B VargaftigC Dumarey

Abstract

Aggregation of washed rabbit platelets by thrombin and by carrageenan is accompanied by the activation of phospholipase A2 and by the synthesis of thromboxanes. Accordingly, aggregation, the accompanying release reaction and the activation of phospholipase are blocked by p-bromophenacyl bromide and by CB 874 (2,3-dibromo (4'-cyclohexyl-3'-chloro)-phenyl-4-oxo-butyric acid), two recognized inhibitors of the enzyme. Since these two reagents also inhibit aggregation and the release reaction induced by thrombin and by carrageenan on washed human platelets, it might have been anticipated that the mechanisms of aggregation of the platelets from the two species are similar. Nevertheless, no thromboxanes A2 or B2, nor activation of phospholipase A2 could be demonstrated with the use of carrageenan on human platelets, under conditions where thrombin was effective. It is concluded that carrageenan activates the human platelets by phospholipase A2- and thromboxane A2-independent mechanisms, and that the inhibitors of phospholipase A2 may block platelet functions by mechanisms other than inhibition of the expected enzyme.

References

Mar 1, 1979·The Journal of Pharmacy and Pharmacology·R M McMillanJ L Gordon
Sep 1, 1979·The Journal of Pharmacy and Pharmacology·E ValleeJ F Delahayes
Jun 1, 1975·The American Journal of Physiology·J F MustardM A Packham
Jan 1, 1979·Thrombosis Research·G KindnessJ Boyd
Jun 28, 1979·Nature·M ChignardJ Benveniste
Feb 23, 1976·Biochimica Et Biophysica Acta·T K BillsM J Silver
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Jul 1, 1970·British Journal of Haematology·N G ArdlieJ F Mustard
May 1, 1964·The Journal of Clinical Investigation·R H ASTER, J H JANDL

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Citations

Oct 26, 2001·Biochemical and Biophysical Research Communications·S A HarveyM S Olson
Oct 27, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·D OlthoffD Kunze
Jan 1, 1982·The International Journal of Biochemistry·M FujiiK Koga
Apr 22, 1987·Biochimica Et Biophysica Acta·M W Collison, J A Thomas
Mar 1, 1984·Prostaglandins, Leukotrienes, and Medicine·N Simberg
Feb 1, 1993·British Journal of Haematology·M HatmiB B Vargaftig
Feb 15, 1981·Biochemical Pharmacology·B B VargaftigJ Benveniste

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