Carrageenan-induced inflammation promotes ROS generation and neutrophil extracellular trap formation in a mouse model of peritonitis

European Journal of Immunology
Cristiane R BarthMárcio V F Donadio

Abstract

Neutrophil extracellular traps (NETs) are a combination of DNA fibers and granular proteins, such as neutrophil elastase (NE). NETs are released in the extracellular space in response to different stimuli. Carrageenan is a sulfated polysaccharide extracted from Chondrus crispus, a marine algae, used for decades in research for its potential to induce inflammation in different animal models. In this study, we show for the first time that carrageenan injection can induce NET release in a mouse model of acute peritonitis. Carrageenan induced NET release by viable neutrophils with NE and myeloperoxidase (MPO) expressed on DNA fibers. Furthermore, although this polysaccharide was able to stimulate reactive oxygen species (ROS) generation by peritoneal neutrophils, NADPH oxidase derived ROS were dispensable for NET formation by carrageenan. In conclusion, our results show that carrageenan-induced inflammation in the peritoneum of mice can induce NET formation in an ROS-independent manner. These results may add important information to the field of inflammation and potentially lead to novel anti-inflammatory agents targeting the production of NETs.

References

Feb 1, 1972·The Journal of Pharmacy and Pharmacology·M Di Rosa
Jul 1, 1973·Proceedings of the Society for Experimental Biology and Medicine·R VinegarJ L Selph
Mar 6, 2004·Science·Volker BrinkmannArturo Zychlinsky
Aug 11, 2004·The Journal of Biological Chemistry·Sibylla MartinelliShida Yousefi
Mar 22, 2006·Cellular Microbiology·Constantin F UrbanArturo Zychlinsky
Jan 11, 2007·The Journal of Cell Biology·Tobias A FuchsArturo Zychlinsky
Jan 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Indira NeeliMarko Radic
Jul 18, 2009·Cell Death and Differentiation·S YousefiH U Simon
Jan 1, 2009·Journal of Innate Immunity·David ErmertVolker Brinkmann
Jan 1, 2009·Journal of Innate Immunity·Sonja OehmckeHeiko Herwald
May 5, 2010·Proceedings of the National Academy of Sciences of the United States of America·Abdul HakkimArturo Zychlinsky
Oct 27, 2010·The Journal of Cell Biology·Venizelos PapayannopoulosArturo Zychlinsky
Nov 26, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Florian H PilsczekPaul Kubes
Jan 19, 2011·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D O GonçalvesG S B Viana
Jun 30, 2011·AIDS Patient Care and STDs·Lisa B Hightow-WeidmanUNKNOWN YMSM of Color SPNS Initiative Study Group
Nov 3, 2011·Journal of Thrombosis and Haemostasis : JTH·A BrillDenisa D Wagner
Jul 28, 2012·Critical Care : the Official Journal of the Critical Care Forum·Wei MengTim T Lögters
Dec 3, 2013·Annals of the Rheumatic Diseases·Nishant Dwivedi, Marko Radic
Aug 3, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lingtao LuoHenrik Thorlacius
Nov 27, 2014·Clinical and Experimental Pharmacology & Physiology·Przemyslaw RzodkiewiczMagdalena Bujalska-Zadrozny
Dec 3, 2014·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Eniko BerkesMona Saffarzadeh

❮ Previous
Next ❯

Citations

Dec 23, 2016·Microbiology and Immunology·Xiaohuan ZhangYan Hu
Feb 1, 2017·European Journal of Endocrinology·Namhee KimHyun Kyung Kim
Jul 26, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monoranjan Boro, Kithiganahalli Narayanaswamy Balaji
Jun 11, 2019·Journal of Cellular Physiology·Josiane Silva SilveiraAline Andrea da Cunha
Jun 18, 2019·Journal of the Science of Food and Agriculture·Laís Ferraz BritoLucas Miranda Marques
May 20, 2020·Journal of Cellular and Molecular Medicine·Jie ZengChao-Jie Zhang
Jul 19, 2019·Frontiers in Veterinary Science·Pia Martiny, Robert Goggs
Aug 7, 2019·Biomolecules·Nina Sanina
Dec 19, 2017·Cell and Tissue Research·Weronika Ortmann, Elzbieta Kolaczkowska
Aug 30, 2019·Current Pharmaceutical Design·Dilshad QureshiKunal Pal
Oct 30, 2020·International Journal of Molecular Sciences·Andrey V MarkovEvgeniya B Logashenko
Oct 30, 2020·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Silvio Almeida JuniorRicardo Andrade Furtado
Jan 29, 2021·Journal of Ethnopharmacology·Guilherme Venâncio SímaroJairo Kenupp Bastos
Mar 2, 2021·Journal of Toxicology and Environmental Health. Part a·Vivianne Rodrigues AmorimPaulo Michel Pinheiro Ferreira
Oct 6, 2017·Archives of Oral Biology·Bruna GenariSusana Maria Werner Samuel
Jun 20, 2021·Comprehensive Reviews in Food Science and Food Safety·Fang LiuRobert W Li
Aug 13, 2021·Wiener medizinische Wochenschrift·Anton TkachenkoOksana Nakonechna

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here