Carrageenan polysaccharides and oligosaccharides with distinct immunomodulatory activities in murine microglia BV-2 cells

International Journal of Biological Macromolecules
Lianzhong AiGuangqiang Wang

Abstract

This study was to investigate the anti-inflammatory activities in vitro of various carrageenans (Car) fractions (κ-, ι-, and λ-types) with well characterized molecular properties, using murine microglia BV-2 cell line treated with lipopolysaccharide (LPS) as model. It is indicated that pretreatments with the oligosaccharide fractions from κ- or ι-carrageenan acid hydrolysates (κ- and ι-CarAOS, respectively) at 125-500 μg/mL significantly and dose-dependently decreased the levels of tumor necrosis factor α (TNF-α) secreted from LPS-treated BV-2 cells, showing promisingly anti-inflammatory effects. Differently, pretreatments of most of polymeric carrageenans at 250-500 μg/mL significantly increased the TNF-α level, implying the co-inflammatory effects with LPS. The co-inflammatory effectiveness of pure carrageenans at 125 μg/mL was notable for λ-Car, followed by ι-Car, and insignificantly for κ-Car. Generally, cytokine TNF-α was a more sensitive biomarker to the presence of carrageenans than was the IL-6. The TNF-α level varied greatly at a low carrageenan concentration (125 μg/mL) and high polymer percentage (e.g. purified κ- and ι-Car). Conclusively, the anti-inflammatory effects on LPS-treated BV-2 cells could be attenuated by...Continue Reading

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Citations

Oct 14, 2019·Journal of Biomedical Materials Research. Part a·Eduardas CicinskasAlexandra A Kalitnik
Dec 2, 2020·Marine Drugs·Yuliya KhrunykHermann Ehrlich
Dec 16, 2020·International Journal of Biological Macromolecules·Xiaona SunChunqing Ai
May 22, 2021·International Journal of Biological Macromolecules·Dongying ZhaoYongming Bao
Sep 9, 2020·Food Hydrocolloids·Katsuyoshi Nishinari, Yapeng Fang

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