Carrier-bound nonallergenic Der p 2 peptides induce IgG antibodies blocking allergen-induced basophil activation in allergic patients.

Allergy
K-W ChenS Vrtala

Abstract

More than 90% of house dust mite-allergic patients are sensitized to the major Dermatophagoides pteronyssinus allergen, Der p 2. The aim of this study was to develop and characterize an allergy vaccine based on carrier-bound Der p 2 peptides, which should allow reducing IgE- and T-cell-mediated side-effects during specific immunotherapy (SIT). Five Der p 2 peptides (P1-P5) were synthesized and analyzed regarding IgE reactivity and allergenic activity. Lymphoproliferative and cytokine responses induced with Der p 2 and Der p 2 peptides were determined in peripheral blood mononuclear cells from mite-allergic patients. Der p 2-specific IgG antibodies induced with carrier-bound Der p 2 peptides in mice and rabbits were tested for their capacity to inhibit IgE binding and basophil activation in allergic patients. Of five overlapping peptides (P1-P5) covering the Der p 2 sequence, two peptides (P2 and P4) were identified, which showed no relevant IgE reactivity, allergenic activity, and induced lower Der p 2-specific T-cell activation than Der p 2. However, when coupled to a carrier, P2 and P4 induced Der p 2-specific IgG antibodies in animals, which inhibited allergic patients' IgE binding to the allergen and allergen-induced basoph...Continue Reading

References

Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S KorematsuN Minato
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·W L OldfieldM Larché
Aug 21, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M FockeR Valenta
Apr 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ines SwobodaSusanne Spitzauer
Jun 11, 2002·Journal of Molecular Biology·U DerewendaD C Benjamin
Jul 12, 2002·The Journal of Allergy and Clinical Immunology·Alexander W HauswirthPeter Valent
Oct 10, 2002·International Archives of Allergy and Immunology·Wayne R ThomasRichard M O'Brien
Nov 12, 2003·The Journal of Allergy and Clinical Immunology·Petra A WachholzStephen R Durham
Apr 15, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G PittnerR Valenta
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·V NiederbergerR Valenta
May 4, 2005·The Journal of Allergy and Clinical Immunology·Jürgen ReisingerVerena Niederberger
Jul 26, 2006·International Archives of Allergy and Immunology·R J J van NeervenP A Würtzen
Oct 6, 2006·The New England Journal of Medicine·Peter S CreticosUNKNOWN Immune Tolerance Network Group
Feb 14, 2008·The Journal of Allergy and Clinical Immunology·Raffaela CampanaRudolf Valenta
Jun 20, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A PurohitO Cromwell
Nov 13, 2008·The Journal of Allergy and Clinical Immunology·Gabrielle PauliHans-Jorgen Malling
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Johanna EdlmayrRudolf Valenta
Mar 10, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M FockeR Valenta
Mar 18, 2011·The Journal of Allergy and Clinical Immunology·Katarzyna NiespodzianaRudolf Valenta
Apr 26, 2011·The Journal of Allergy and Clinical Immunology·Teresa E TwarochRudolf Valenta

❮ Previous
Next ❯

Citations

Mar 29, 2014·Current Opinion in Otolaryngology & Head and Neck Surgery·Anna J Knisely, Stella Lee
Mar 15, 2016·Immunotherapy·Peter A WürtzenPeter S Andersen
May 30, 2012·Journal of Internal Medicine·R ValentaM van Hage
Jul 26, 2014·Allergy·F BrazaS Brouard
Apr 24, 2012·Current Opinion in Immunology·Birgit Linhart, Rudolf Valenta
Feb 15, 2015·The Journal of Allergy and Clinical Immunology·Gregory BouchaudAntoine Magnan
Feb 11, 2015·Applied Microbiology and Biotechnology·Chunqing AiWei Chen
Aug 6, 2013·Methods : a Companion to Methods in Enzymology·Susanne VrtalaWayne R Thomas
Aug 14, 2012·Current Opinion in Allergy and Clinical Immunology·Margarete Focke-Tejkl, Rudolf Valenta
Oct 12, 2014·Current Opinion in Allergy and Clinical Immunology·Marek Jutel, Cezmi A Akdis
Sep 30, 2016·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Carla OseroffAlessandro Sette
Apr 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Srinita BanerjeeSusanne Vrtala
Jun 11, 2018·Current Allergy and Asthma Reports·Mirela CurinRudolf Valenta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergies & Environmental Factors

Environmental factors are strongly associated with the prevalence of allergies and are an increasing health concern worldwide. Discover the latest research on Allergies and Environmental Factors here.

Basophils

Basophils are myeloid cells with a high affinity IgE receptor and is involved in inflammatory responses during allergy. Discover the latest research on Basophils here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.