PMID: 7536316Jan 30, 1995Paper

Carrier-mediated uptake and release of histamine by cultured rat cerebral endothelial cells

Neuroscience Letters
Z HusztiF Joó

Abstract

The present study demonstrates that histamine could be taken up by and released from endothelial cells of brain capillaries. Incubation of cultured endothelial cells, with low (0.01-0.50 microM) concentrations of [3H]histamine, resulted in a rapid uptake of the amine. The uptake was saturable, Na(+)-dependent and yielded an apparent Km 0.3 +/- 0.02 microM and a Vmax 4.6 +/- 0.04 pmol/mg protein per min. After a 10-min incubation in a histamine-free medium, about 65% of [3H]histamine was released from the cells. Na(+)-deprivation and high K+, as well as the treatment of the cells with ouabain affected the release, resulting in significantly higher rates of the efflux. The ability of cerebral endothelial cells to take up histamine from both luminal and abluminal sides but to release it mainly luminally, may function as an important mechanism to protect the neural tissue from the harmful effects of this endogenous mediator of inflammation.

References

Jul 1, 1991·Journal of Neuroscience Research·E DuxP H Chan
Jan 1, 1992·Journal of Neurochemistry·F Joó
Mar 1, 1989·Journal of the Neurological Sciences·S MohantyY Olsson
May 1, 1987·Neuropathology and Applied Neurobiology·F Joó
Jan 1, 1982·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·P M Gross
Apr 1, 1981·Agents and Actions·F JoóZ Mezei

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Citations

Oct 20, 2001·Behavioural Brain Research·L Fernández-Novoa, R Cacabelos
Mar 10, 2000·Journal of Chemical Neuroanatomy·P PanulaM Lintunen
Mar 17, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K KarlstedtP Panula
Jun 1, 1995·Pharmacology & Toxicology·Z Huszti, I Balogh
Mar 13, 2003·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Béla KisYoichi Ueta
May 16, 2012·Neurochemistry International·Katja Perdan-PirkmajerMojca Kržan
Aug 11, 2010·Neuroscience and Biobehavioral Reviews·David T MarcKelly L Olson
Dec 1, 2001·Biochemical Pharmacology·Z HusztiA M Deli

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