Cartilage-derived morphogenetic proteins enhance the osteogenic protein-1-induced osteoblastic cell differentiation of C2C12 cells

Journal of Cellular Physiology
Lee-Chuan C YehJohn C Lee

Abstract

Previous studies have shown that osteogenic protein-1 (OP-1; also known as BMP-7) induces differentiation of the pluripotent mesenchymal cell line C2C12 into osteoblastic cells. OP-1 also alters the steady-state levels of messenger RNA (mRNA) encoding for the cartilage-derived morphogenetic proteins (CDMPs) in C2C12 cells. In the present study, the effects of exogenous CDMPs on bone cell differentiation induced by OP-1 in C2C12 cells were examined. Exogenous CDMP-1, -2, and -3 synergistically and dose-dependently enhanced OP-1 action in stimulating alkaline phosphatase (AP) activity and osteocalcin (OC) mRNA expression. AP staining studies revealed that the combination of OP-1 and CDMP enhanced OP-1 action by stimulating those cells that had responded to OP-1 and not by activating additional cells. The combination did not change the mRNA expression of the BMPs and their receptors. CDMP-1 enhanced the suppression of the OP-1-induced expression of the myogeneic differentiation regulator MyoD. CDMP-1 and OP-1 alone stimulated Smad5 protein expression, but the combination of OP-1 and CDMP-1 stimulated synergistically Smad5 protein expression. Thus, one mechanism of the observed synergy involved enhancement of the induced Smad5 prot...Continue Reading

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Citations

May 3, 2005·Leukemia Research·Ephraim Philip Lansky, Daniel D Von Hoff
Mar 27, 2009·Tissue Engineering. Part a·Blanca San MiguelFranz E Weber
Sep 18, 2008·International Journal of Biological Sciences·Lisa A WilliamsAshish D Diwan
Feb 26, 2009·International Journal of Biological Sciences·Bojiang ShenAshish D Diwan
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Feb 2, 2010·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Tara AghalooZhiyuan Zhang
Dec 17, 2014·The Journal of Oral Implantology·Fabiano Ribeiro CiranoLuiz Antônio Pugliesi Alves Lima
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May 30, 2008·Clinical Orthopaedics and Related Research·Zhinong HuangStuart B Goodman
Mar 11, 2005·Journal of Cellular Biochemistry·Lee-Chuan C YehJohn C Lee

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