Cartilage mechanical response under dynamic compression at physiological stress levels following collagenase digestion

Annals of Biomedical Engineering
Seonghun ParkGerard A Ateshian

Abstract

The objective of this study was to test the hypothesis that enzymatic degradation by collagenase significantly reduces dynamic moduli and increases compressive strains of bovine articular cartilage under physiological compressive stress levels and loading frequencies. Twenty-seven distal femoral cartilage plugs (3 mm diameter) were loaded in a custom apparatus under load control, with a load up to 40 N and loading frequencies of 0.1, 1, 10, and 40 Hz, before and after incubation in physiological buffered saline containing various concentrations of collagenase (0, 2, and 10 U/mL). Collagenase digestion reduced the equilibrium Young's modulus by 49% with 2 U/mL and 61% with 10 U/mL, while the decrease in dynamic modulus at 40 Hz was in the range of 13-20% with 2 U/mL and 24-33% with 10 U/mL, relative to respective controls. The amplitudes of dynamic compressive strains increased from 22 +/- 6% to 26 +/- 8% at 0.1 Hz and 9.6 +/- 3.3% to 13.5 +/- 3.2% at 40 Hz, with 10 U/mL collagenase. This experimental study serves to confirm that collagen contributes significantly to the dynamic compressive properties of cartilage, by demonstrating that collagenase digestion impairs these properties, under stress amplitudes and frequencies which...Continue Reading

References

Jan 1, 1977·Acta Orthopaedica Scandinavica·L S MatthewsJ A Henke
May 28, 1976·Biochimica Et Biophysica Acta·G E KempsonP H Horsfield
May 1, 1990·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M B SchmidtD R Eyre
Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R L SahJ D Sandy
Nov 1, 1967·Clinica Chimica Acta; International Journal of Clinical Chemistry·H Stegemann, K Stalder
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Feb 1, 1995·Arthritis and Rheumatism·L J BonassarA J Grodzinsky
Nov 1, 1993·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·W ZhuD R Eyre
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·L E FreedG Vunjak-Novakovic
Jan 7, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M J KääbH P Nötzli
Mar 12, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·G Vunjak-NovakovicL E Freed
Jul 9, 1999·Journal of Biomechanical Engineering·J SoulhatA Shirazi-Adl
Aug 11, 2000·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·K W LiR L Sah
Aug 31, 2001·Biochemical and Biophysical Research Communications·K J GoochL E Freed
Sep 11, 2002·Annals of Biomedical Engineering·Mark R DiSilvestro, Jun-Kyo Francis Suh
Aug 2, 2003·Journal of Biomechanics·Mikko S LaasanenJukka S Jurvelin
Nov 15, 2003·Journal of Biomechanics·Seonghun ParkGerard A Ateshian
Mar 31, 2005·Journal of Biomechanical Engineering·Ines M BasaloGerard A Ateshian
Jul 4, 2006·Biochimica Et Biophysica Acta·Xiaohong BiNancy Pleshko Camacho

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Citations

Mar 30, 2012·Biomedical Materials·Isaac E EricksonRobert L Mauck
Jun 10, 2010·BMC Developmental Biology·Mark C van TurnhoutJohan L van Leeuwen
Oct 12, 2010·BMC Musculoskeletal Disorders·Matthew SzarkoJohn Ea Bertram
Mar 23, 2011·Clinical Orthopaedics and Related Research·Isaac E EricksonRobert L Mauck
Feb 2, 2010·Journal of Biomechanics·Ana BarbirJames C Iatridis
Apr 8, 2015·Journal of Tissue Engineering and Regenerative Medicine·Shibu ChameettachalSourabh Ghosh
May 29, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bhavana MohanrajRobert L Mauck
Jun 3, 2017·Annals of Biomedical Engineering·A GkousioudiL G Alexopoulos
Sep 23, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Guebum HanCorinne R Henak

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