Cartilage regeneration using arthroscopic flushing fluid-derived mesenchymal stem cells encapsulated in a one-step rapid cross-linked hydrogel

Acta Biomaterialia
Jun LiGuangqian Zhou

Abstract

Many attempts have been made to repair articular cartilage defects, including mesenchymal stem cell (MSC)-based tissue engineering strategies. Although this approach shows promise, optimizing MSC sources and their delivery is challenging. This study was designed to test the feasibility of using MSCs found in the human arthroscopic flushing fluid (AFF) for cartilage regeneration, by incorporating them into a newly developed one-step rapid cross-linking hyper-branched polyPEGDA/HA hydrogel. AFF-MSCs were isolated from the original intra-articular flushing fluid of 10 patients prior to arthroscopic procedures. The hydrogel was fabricated with hyper-branched polyPEGDA and thiolated hyaluronic acid (HA). In vitro assays demonstrated that AFF-MSCs possessed the typical MSC morphology and phenotype, and maintained chondrogenic differentiation properties when encapsulated within the hydrogel. The AFF-MSC/hydrogel composite could significantly repair full-thickness cartilage defects generated in a rat model after 8 weeks of implantation; smooth cartilage was formed with evidence of hyaline cartilage formation. These data suggest that human AFF-MSCs are a novel and abundant MSC source that have high therapeutic value for cartilage regene...Continue Reading

Citations

Dec 6, 2019·Frontiers in Bioengineering and Biotechnology·Kendrick ToWasim Khan
Jul 31, 2020·Tissue Engineering. Part B, Reviews·Wassif KabirPeter F M Choong
Feb 26, 2021·Stem Cells International·Shuangpeng JiangQuanyi Guo
Dec 11, 2020·Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases·Robert W IkeWilliam J Arnold
May 21, 2021·Colloids and Surfaces. B, Biointerfaces·Erlane de Sousa AraújoAnderson Oliveira Lobo
Jul 30, 2021·International Journal of Rheumatic Diseases·Robert W Ike, Kenneth C Kalunian
May 14, 2020·The Journal of Knee Surgery·William FangC Thomas Vangsness

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