Cartilage tissue formation through assembly of microgels containing mesenchymal stem cells

Acta Biomaterialia
Fanyi LiJessica E Frith

Abstract

Current clinical approaches to treat articular cartilage degeneration provide only a limited ability to regenerate tissue with long-term durability and functionality. In this application, injectable bulk hydrogels and microgels containing stem cells can provide a suitable environment for tissue regeneration. However insufficient cell-cell interactions, low differentiation efficiency and poor tissue adhesion hinder the formation of high-quality hyaline type cartilage. Here, we have designed a higher order tissue-like structure using injectable cell-laden microgels as the building blocks to achieve human bone marrow-derived mesenchymal stem cell (hBMSC) long-term maintenance and chondrogenesis. We have demonstrated that a 4-arm poly(ethylene glycol)-N-hydroxysuccinimide (NHS) crosslinker induces covalent bonding between the microgel building blocks as well as the surrounding tissue mimic. The crosslinking process assembles the microgels into a 3D construct and preserves the viability and cellular functions of the encapsulated hBMSCs. This assembled microgel construct encourages upregulation of chondrogenic markers in both gene and glycosaminoglycan (GAG) expression levels. In addition, the regenerated tissue in the assembled micr...Continue Reading

Citations

Apr 17, 2020·Frontiers in Bioengineering and Biotechnology·Bingbing XuJia-Kuo Yu
Oct 28, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lijing TengLi Ren
Jul 25, 2020·Journal of Materials Science. Materials in Medicine·André F GirãoPaula A A P Marques
Mar 14, 2020·International Journal of Molecular Sciences·Lixia HuangGuang Yang
Oct 15, 2019·Annals of the New York Academy of Sciences·Mohammed M BenmassaoudSebastián L Vega
Feb 17, 2021·ACS Biomaterials Science & Engineering·Victoria G MuirJason A Burdick
Mar 13, 2021·Advanced Healthcare Materials·Clara R CorreiaJoão F Mano
Apr 13, 2021·Advanced Functional Materials·Alexander S CaldwellKristi S Anseth
Jan 1, 2020·Nature Reviews. Materials·Andrew C DalyJason A Burdick
Jul 4, 2021·Advanced Healthcare Materials·Maria C GomesJoão F Mano
Aug 7, 2021·Frontiers in Bioengineering and Biotechnology·Shuai ChenVahid Serpooshan
Nov 26, 2020·Biomacromolecules·Surakshya ShresthaJessica E Frith
Jan 19, 2021·ACS Biomaterials Science & Engineering·Anisha B SuraiyaAnn P Chidgey
Dec 29, 2020·Chemical Reviews·Victoria G Muir, Jason A Burdick

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