CASC15 contributes to proliferation and invasion through regulating miR-766-5p/ KLK12 axis in lung cancer

Cell Cycle
Yong BaiHeying Chu

Abstract

Long non-coding RNAs (lncRNAs) are key mediators of cancer. The dysregulation of a lncRNA, CASC15, has been linked to several cancers, except lung cancer. Here, the aim of the study was to explore the role and mechanism of CASC15 in lung cancer regulation, with the focus on its interaction with a potential target, microRNA-766-5p (miR-766-5p) and an oncogene, kallikrein-related peptidase 12 (KLK12). Quantitative real-time PCR (qRT-PCR) was used to assess levels of CASC15, miR-766-5p and KLK12 in lung cancer tissues or cells. Western blot analysis was used to detect KLK12 protein expression. Ectopic expression of CASC15 was induced by a lentiviral system. CCK-8 and transwell assays were used to evaluate lung cancer cell proliferation and invasion, respectively. The interaction among CASC15, miR-766-5p and KLK12 was investigated by bioinformatical analysis and luciferase assay. In lung cancer tissue and cells, CASC15 was upregulated, while miR-766-5p was downregulated. Overexpression of CASC15 promoted lung cancer cell proliferation and invasion. A negative correlation was found between CASC15 and miR-766-5p levels. Overexpression of miR-766-6p reversed the cancer-promoting role of CASC15 in lung cancer cells, which was mediated ...Continue Reading

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Citations

Dec 18, 2020·Technology in Cancer Research & Treatment·Yongqin CaiHonggang Wang
Jan 13, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinyu GuHaihong Zhu
Jan 8, 2021·Journal of Experimental & Clinical Cancer Research : CR·Jianyong SunLei Wang
Jun 27, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Haihong ZhouLili Cui

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Methods Mentioned

BETA
transfect
PCR
Assay
electrophoresis
transfection

Software Mentioned

BLAST

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