Cascade Signal Amplification Based on Copper Nanoparticle-Reported Rolling Circle Amplification for Ultrasensitive Electrochemical Detection of the Prostate Cancer Biomarker

ACS Applied Materials & Interfaces
Ye ZhuWei Jiang

Abstract

An ultrasensitive and highly selective electrochemical assay was first attempted by combining the rolling circle amplification (RCA) reaction with poly(thymine)-templated copper nanoparticles (CuNPs) for cascade signal amplification. As proof of concept, prostate specific antigen (PSA) was selected as a model target. Using a gold nanoparticle (AuNP) as a carrier, we synthesized the primer-AuNP-aptamer bioconjugate for signal amplification by increasing the primer/aptamer ratio. The specific construction of primer-AuNP-aptamer/PSA/anti-PSA sandwich structure triggered the effective RCA reaction, in which thousands of tandem poly(thymine) repeats were generated and directly served as the specific templates for the subsequent CuNP formation. The signal readout was easily achieved by dissolving the RCA product-templated CuNPs and detecting the released copper ions with differential pulse stripping voltammetry. Because of the designed cascade signal amplification strategy, the newly developed method achieved a linear range of 0.05-500 fg/mL, with a remarkable detection limit of 0.020 ± 0.001 fg/mL PSA. Finally, the feasibility of the developed method for practical application was investigated by analyzing PSA in the real clinical hu...Continue Reading

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Citations

Aug 18, 2016·ACS Applied Materials & Interfaces·Bei-Bei KouRuo Yuan
Feb 14, 2017·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Wenping ZhuYahong Chen
Jan 6, 2018·Cancers·Shin-Ichiro HoriJiehua Zhou
Jul 13, 2019·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Chung-An ChenKevin C-W Wu
Feb 11, 2020·Chemical Communications : Chem Comm·Xuan-Xuan ZhangWei Ma
Jun 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mengqi ZouYun Xiang
Sep 14, 2018·Pharmaceuticals·Dario Ruiz CiancioMaría Belén Mestre
Nov 28, 2019·Sensors·Alba Iglesias-MayorAlfredo de la Escosura-Muñiz
Sep 11, 2020·Biosensors·Lasangi DhanapalaJames F Rusling
Nov 8, 2017·Mikrochimica Acta·Dominika DamborskaJan Tkac
Aug 23, 2020·Sensors·William S Skinner, Keat Ghee Ong
Feb 7, 2019·Engineering in Life Sciences·Zahra Akbari JonousMeisam Omidi
Sep 20, 2020·Sensors·Laura Cid-BarrioJosé Manuel Costa-Fernández
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May 29, 2021·Analytical Methods : Advancing Methods and Applications·Wenwen MengYuzhong Zhang
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Dec 22, 2017·ACS Applied Materials & Interfaces·Bo TianMattias Strömberg
Dec 16, 2017·ACS Applied Materials & Interfaces·Min LiLihua Wang

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