Casein kinase activity in etiolated Cucumis sativus cotyledons

Plant Biology
Dolores VidalEsther Simón

Abstract

Two calcium- and light-dependent protein kinases have been reported in etiolated Cucumis sativus cotyledons (Vidal et al. 2007). In the present work, we studied casein kinase (CK) activity in etiolated cucumber cotyledons of in-gel and in vitro kinase assays, using specific CK inhibitors, and ATP and GTP as phosphate donors. Two proteins with CK activity were detected in both casein gels and autophosphorylation assays. One of them, with a molecular mass of approximately 36 kDa, showed biochemical CK1 characteristics: it was inhibited by specific CK1 inhibitors and only used ATP as phosphate donor. The second, with a molecular mass of approximately 38 kDa, had CK2 characteristics; it used both ATP and GTP as phosphate donors, was inhibited by all specific CK2 inhibitors, and was recognized by a polyclonal antibody directed against the alpha catalytic subunit of a CK2 from tobacco. The kinase activity of the CK2 detected in etiolated cucumber cotyledons showed circadian rhythmicity in both in vitro and in-gel casein phosphorylation and in autophosphorylation assays. Thus, our results suggest that the CK2 of approximately 38 kDa could be related to the circadian oscillator of C. sativus cotyledons.

References

Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J RowlesM H Cobb
Sep 24, 1990·Biochimica Et Biophysica Acta·L A Pinna
Feb 15, 1986·Analytical Biochemistry·R L GeahlenM L Harrison
Sep 15, 1995·The Journal of Biological Chemistry·P R Graves, P J Roach
Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Allende, C C Allende
Jul 1, 1993·The Biochemical Journal·L J Klimczak, A R Cashmore
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·S SuganoE M Tobin
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·S SuganoE M Tobin
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M C EspunyaM C Martínez
Dec 3, 1999·Nature Structural Biology·K NiefindD Schomburg
Dec 21, 2000·Biochimica Et Biophysica Acta·H L HsiehS J Roux
Oct 18, 2001·Protein Science : a Publication of the Protein Society·R BattistuttaL A Pinna
Nov 26, 2002·Nature·Jui-Ming LinRavi Allada
Feb 4, 2003·Nature Neuroscience·Bikem AktenF Rob Jackson
Feb 26, 2003·Nature Neuroscience·Justin Blau
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Oct 11, 2003·The Plant Journal : for Cell and Molecular Biology·Wei LiuHong-Wei Xue
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xavier DanielElaine M Tobin
Nov 10, 2004·Journal of Biological Rhythms·Patrice A Salomé, C Robertson McClung
Jan 28, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Mary Ellen K Olsten, David W Litchfield
Feb 22, 2005·Cellular Signalling·Uwe KnippschildMartin Stöter
Jan 24, 2006·Cellular and Molecular Life Sciences : CMLS·E TibaldiL A Pinna
Apr 6, 2006·The Plant Cell·C Robertson McClung
Dec 23, 2006·Phytochemistry·Dolores VidalEsther Simón
Feb 1, 2007·Plant, Cell & Environment·Carlos T HottaAlex A R Webb
Jul 31, 2007·The Plant Journal : for Cell and Molecular Biology·Sergi Portolés, Paloma Más

❮ Previous
Next ❯

Citations

Dec 12, 2012·Plant Physiology and Biochemistry : PPB·Fagua Alvarez-FlórezEsther Simón
Jul 15, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Elsa MatthusMichael Frei

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.