PMID: 8592152Feb 1, 1996Paper

Casein kinase II phosphorylates the neural cell adhesion molecule L1

Journal of Neurochemistry
E V WongV Lemmon

Abstract

L1 is an axonal cell adhesion molecule found primarily on projection axons of both the CNS and PNS. It is a phosphorylated membrane-spanning glycoprotein that can be immunoprecipitated from rat brain membranes in association with protein kinase activities. Western blot analysis demonstrates that casein kinase II (CKII), a ubiquitous serine/threonine kinase enriched in brain, is present in these immunoprecipitates. CKII preparations partially purified from PC12 cells are able to phosphorylate recombinant L1 cytoplasmic domain (L1CD), which consists of residues 1,144-1,257. Using these as well as more highly purified kinase preparations, phosphorylation assays of small peptides derived from the L1CD were performed. CKII was able to phosphorylate a peptide encompassing amino acids (aa) 1,173-1,185, as well as a related peptide representing an alternatively spliced nonneuronal L1 isoform that lacks aa 1,177-1,180. Both peptides were phosphorylated with similar kinetic profiles. Serine to alanine substitutions in these peptides indicate that the CKII phosphorylation site is at Ser1,181. This is consistent with experiments in which L1CD was phosphorylated by these kinase preparations, digested, and the radiolabeled fragments sequence...Continue Reading

Citations

Jan 1, 1996·Cell Motility and the Cytoskeleton·S M Burden-Gulley, V Lemmon
May 20, 1998·Molecular Neurobiology·K C BreenF D Hayes
Dec 16, 1998·The Journal of Cell Biology·S KunzP Sonderegger
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Kenwrick, P Doherty
Aug 10, 2000·Journal of Neurobiology·K E Long, V Lemmon
Sep 26, 2008·Pakistan Journal of Biological Sciences : PJBS·Mostafa Rezaei-TaviraniPourkhoshbakht Yousof
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Heike RebholzPaul Greengard
Dec 26, 2015·Biology·Mirka HomrichSimone Diestel
Jul 1, 1997·Journal of Neuroscience Research·H Kamiguchi, V Lemmon
May 1, 1999·The Journal of Biological Chemistry·C F BearerG Cheng
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
Nov 19, 2005·The Journal of Biological Chemistry·Eugene TomblerMaría A Diversé-Pierluissi
Jan 27, 2007·Journal of Neuroscience Research·Asuka Nakata, Hiroyuki Kamiguchi
Jan 27, 2007·Journal of Neurochemistry·Gabriele Loers, Melitta Schachner
Jun 18, 2017·Molecular and Cellular Biochemistry·Selami DemirciFikrettin Şahin
Jul 26, 1996·The Journal of Biological Chemistry·E V WongV Lemmon
Mar 15, 1997·Journal of Neuroscience Research·A H ZischE B Pasquale
Dec 23, 1999·The Journal of Biological Chemistry·A W SchaeferV Lemmon
Jan 29, 2011·American Journal of Medical Genetics. Part a·Malin RehnbergCecilia Gunnarsson
Jan 8, 2014·The Journal of Cell Biology·Victoria BulatJan Pielage
Oct 18, 2000·The Journal of Biological Chemistry·K E LongV Lemmon
Apr 10, 2009·The Biochemical Journal·Lissa R HerronFrank J Gunn-Moore
Mar 26, 2010·Molecular Biology of the Cell·Maxine M ChenSteve Silletti
Nov 8, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaowei DouUNKNOWN Collaborative Initiative on Fetal Alcohol Spectrum Disorders
Jun 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K L Crossin, L A Krushel
May 2, 2003·The Journal of Biological Chemistry·Mona BuhusiPatricia F Maness
Jun 10, 1998·Molecular and Cellular Neurosciences·B WangS Kenwrick
May 6, 1998·Current Opinion in Neurobiology·T BrümmendorfF G Rathjen
Feb 5, 2000·Progress in Neurobiology·P R Blanquet
Oct 3, 2001·Pediatric Clinics of North America·C F Bearer
Oct 16, 2007·Molecular and Cellular Neurosciences·Roger PocockOliver Hobert

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