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CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes

bioRxiv

May 12, 2014

John AachGeorge M. Church

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Abstract

CRISPR/Cas9 systems enable many molecular activities to be efficiently directed in vivo to user-specifiable DNA sequences of interest, including generation of dsDNA cuts and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of sh...read more

Mentioned in this Paper

DNA Sequence
Gene Knockout Techniques
Neisseria meningitidis
Sphaerobacter thermophilus
Streptococcus pyogenes
Genome
Saccharococcus thermophilus
Site
Genes
Clustered Regularly Interspaced Short Palindromic Repeats
27
80
Paper Details
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  • CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomes

    bioRxiv

    May 12, 2014

    John AachGeorge M. Church

    PMID: 990005074

    DOI: 10.1101/005074

    Abstract

    CRISPR/Cas9 systems enable many molecular activities to be efficiently directed in vivo to user-specifiable DNA sequences of interest, including generation of dsDNA cuts and nicks, transcriptional activation and repression, and fluorescence. CRISPR targeting relies on base pairing of sh...read more

    Mentioned in this Paper

    DNA Sequence
    Gene Knockout Techniques
    Neisseria meningitidis
    Sphaerobacter thermophilus
    Streptococcus pyogenes
    27
    80
    Paper Details
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