CASK is in the mammalian sperm head and is processed during epididymal maturation

Molecular Reproduction and Development
H R BurkinD J Miller

Abstract

Upon adhesion to the zona pellucida or egg extracellular matrix, sperm undergo regulated exocytosis of the acrosomal vesicle. CASK is an adaptor protein that has been implicated in coupling neuronal cell adhesion to regulated exocytosis. In neurons, this scaffolding molecule is associated with several types of transmembrane receptor complexes and connects cell adhesion molecules with ion channels, the actin cytoskeleton, and the cell's exocytotic machinery. We hypothesized CASK might also be an important link between zona pellucida binding and the sperm acrosome reaction. RT-PCR experiments indicated CASK is transcribed in mouse testis. The full size (120 kDa) CASK protein was present in testis from mouse and pig. Immunoblots of mature porcine and murine sperm revealed that the 120 kDa molecule was much less abundant than in testis but the antibody also recognized a group of smaller proteins migrating at 55-65 kDa. Immunofluorescence experiments indicated both the full length and smaller CASK immunoreactive products were found only in the acrosomal region of spermatids and mature sperm and not in other testicular cell types. CASK immunofluorescence was lost following the acrosome reaction. During epididymal maturation, the abun...Continue Reading

References

Aug 17, 1999·The EMBO Journal·I DulubovaJ Rizo
Aug 24, 1999·The Journal of Biological Chemistry·A MaximovI Bezprozvanny
Mar 22, 2000·Biology of Reproduction·R YunesL S Mayorga
Apr 5, 2000·Nature·D S Bredt
Jun 23, 2000·Developmental Biology·J Ramalho-SantosG Schatten
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·M MichautL S Mayorga
Apr 20, 2001·Biochimica Et Biophysica Acta·T C TsengA H Chishti
Oct 18, 2001·The Journal of Biological Chemistry·T Biederer, T C Sudhof
May 15, 2002·The EMBO Journal·Carine BécamelPhilippe Marin

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Citations

Mar 24, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Vincent HyenneMarie-Christine Simmler
Oct 25, 2011·Journal of Cellular Physiology·Rolands G AravindanPatricia A Martin-Deleon
Sep 29, 2009·The International Journal of Biochemistry & Cell Biology·Qizhi Sun, Gregory M Kelly
Jan 5, 2007·Biology of Reproduction·Dolores BussoPatricia S Cuasnicú

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