CASP4/caspase-11 promotes autophagosome formation in response to bacterial infection

Autophagy
Kathrin KrauseAmal O Amer

Abstract

CASP4/caspase-11-dependent inflammasome activation is important for the clearance of various Gram-negative bacteria entering the host cytosol. Additionally, CASP4 modulates the actin cytoskeleton to promote the maturation of phagosomes harboring intracellular pathogens such as Legionella pneumophila but not those enclosing nonpathogenic bacteria. Nevertheless, this non-inflammatory role of CASP4 regarding the trafficking of vacuolar bacteria remains poorly understood. Macroautophagy/autophagy, a catabolic process within eukaryotic cells, is also implicated in the elimination of intracellular pathogens such as Burkholderia cenocepacia. Here we show that CASP4-deficient macrophages exhibit a defect in autophagosome formation in response to B. cenocepacia infection. The absence of CASP4 causes an accumulation of the small GTPase RAB7, reduced colocalization of B. cenocepacia with LC3 and acidic compartments accompanied by increased bacterial replication in vitro and in vivo. Together, our data reveal a novel role of CASP4 in regulating autophagy in response to B. cenocepacia infection.

References

Sep 11, 1992·Journal of Cellular Physiology·A AplinW A Dunn
Dec 1, 1995·The Journal of Cell Biology·Y FengA Wandinger-Ness
Feb 26, 2000·Molecular Biology of the Cell·C BucciB van Deurs
Jun 20, 2003·Cellular Microbiology·Kathryn A RichPaul Webster
May 13, 2004·Journal of Cell Science·Maximiliano G GutierrezMaría I Colombo
Sep 2, 2004·Journal of Cell Science·Stefanie JägerEeva-Liisa Eskelinen
Nov 6, 2004·Science·Ichiro NakagawaTamotsu Yoshimori
Apr 29, 2005·The Journal of Biological Chemistry·Takahide KounoKeiichi Kawano
May 13, 2005·Cellular Microbiology·Amal O Amer, Michele S Swanson
Jul 29, 2005·The Journal of Biological Chemistry·Maya P ByfieldJonathan M Backer
Sep 24, 2005·Proceedings of the National Academy of Sciences of the United States of America·Takahiro NobukuniGeorge Thomas
Feb 24, 2006·The Journal of Biological Chemistry·Cheryl L BirminghamJohn H Brumell
Sep 21, 2006·The Journal of Biological Chemistry·Amal AmerGabriel Núñez
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mohamed LamkanfiGabriel Núñez
Jul 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Adam M BresslerChristopher W Woods
Aug 1, 2008·Molecular & Cellular Proteomics : MCP·Mohamed LamkanfiGabriel Núñez
Mar 7, 2009·The Journal of Biological Chemistry·Phillip A Vanlandingham, Brian P Ceresa
Oct 1, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P Drevinek, E Mahenthiralingam
Jul 20, 2011·Cell & Bioscience·Susann LudwigAria Baniahmad
Oct 18, 2011·Nature·Nobuhiko KayagakiVishva M Dixit
Mar 1, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mikhail A GavrilinAmal O Amer
Oct 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eliseo F CastilloVojo Deretic
Jan 26, 2013·Science·Youssef AachouiEdward A Miao
Apr 9, 2013·Developmental Cell·Bernadette CarrollVania M M Braga

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Citations

Dec 11, 2019·Infection and Immunity·Chanya SrisaowakarnPongsak Utaisincharoen
Aug 2, 2020·Immunological Reviews·Arwa Abu Khweek, Amal O Amer
Sep 24, 2020·International Journal of Molecular Sciences·Savita DeviAndrea Dorfleutner
Jan 15, 2021·Scientific Reports·Shady EstfanousAmal O Amer
Feb 7, 2021·International Journal of Molecular Sciences·Aidan AgnewEmma M Creagh
Nov 20, 2020·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Kaitlin HamiltonAmal O Amer
Nov 15, 2020·Molecular Aspects of Medicine·Kevin P DownsChristian Stehlik
Dec 7, 2021·Frontiers in Immunology·Debabrata ChowdhuryKavitha Subramanian Vignesh

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Methods Mentioned

BETA
GTPase
bronchoalveolar lavage
transmission electron microscopy
lipidation
confocal microscopy
GTPases
immunoprecipitation
deamidation
ELISA
Assay

Software Mentioned

ImageJ

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