Caspase 3 activation during herpes simplex virus 1 infection

Virus Research
Rachel M KraftJohn A Blaho

Abstract

During herpes simplex virus 1 (HSV-1) infection, apoptosis is initiated by immediate early gene transcription and is later modulated by proteins synthesized in infected cells. We have previously shown that procaspase 3 levels are reduced during HSV-1 replication. We now demonstrate that a replication-defective HSV-1 recombinant virus which is incapable of packaging viral DNA into capsids activated caspase 3 but retained the ability to prevent the apoptotic process from killing the infected cells. This implies that HSV-1-dependent apoptosis is not merely a response to abortive infection. Maximum accumulation of the active form of caspase 3 accompanied complete HSV-1-dependent apoptosis. Additionally, caspase 7 was found to be activated during HSV-1-dependent apoptosis. Infected MCF-7 cells which ectopically express caspase 3 underwent more efficient apoptosis than their caspase 3-null parental counterparts, confirming that caspase 3 contributes to HSV-1-dependent apoptosis. However, caspase 3 reconstitution did not make the MCF-7 cells as sensitive as HEp-2 cells to HSV-1-dependent apoptosis, suggesting that other cellular factors may be involved in conferring resistance to this process. These results indicate that caspase 3 act...Continue Reading

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Citations

Oct 18, 2014·Nature Communications·Katelynn A MiloraLiselotte E Jensen
Sep 15, 2007·Journal of Virology·Marie L NguyenJohn A Blaho
Dec 23, 2008·Molecular & Cellular Proteomics : MCP·Enrique SantamaríaFernando J Corrales
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Apr 22, 2016·Journal of Neurovirology·Yael TsalenchuckAmos Panet
Jan 22, 2015·Journal of Neurovirology·Peter G E Kennedy
Jun 8, 2007·The Journal of General Virology·Marie L NguyenJohn A Blaho
Mar 20, 2021·Molecular Therapy Oncolytics·Jayeeta GhoseFlavia Pichiorri

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