Abstract
Apoptosis of nucleated cells is regulated by caspases, a group of cysteine proteases, and is characterized by phosphatidylserine expression on the outer leaflet of the plasma membrane. Reports indicate that platelets contain caspases. However, the role of caspases in platelet function is not well understood. When platelets become activated, they express phosphatidylserine (PS) on the outer leaflet of the plasma membrane. In addition, platelets aggregate when activated. The aims of this study were to determine if caspase inhibition (using the pan-caspase inhibitor zVAD-fmk): (1) decreased PS expression and (2) decreased platelet aggregation following activation. Flow cytometry was used to determine PS expression and a platelet aggregometer was used to assess aggregation. We found that platelets treated with zVAD-fmk significantly decreased both A23187-induced PS exposure (total fluorescence index, TFI: A23187=791.42+/-174; zVAD+A23187=92.97+/-57, p<==0.05) and ADP-induced PS exposure (TFI: ADP=669.24+/-145, zVAD+ADP=174.6+/-151, p<==0.05). Further, treatment with zVAD-fmk significantly decreased ADP-induced platelet aggregation (%: untreated=80+/-1.5, zVAD treated=69+/-3.0, p<==0.05). These results indicate that caspases play a ...Continue Reading
References
Apr 15, 1986·Thrombosis Research·S D Dwyer, K M Meyers
Apr 1, 1986·Thrombosis Research·S D Dwyer, K M Meyers
Mar 1, 1985·Science·H F DvorakJ McDonagh
Jan 1, 1994·Thrombosis Research·E H MurerN J Martinez
Feb 1, 1997·American Journal of Hematology·J N KatopodisY S Ahn
Jul 1, 1997·Thrombosis Research·B WalkowiakL Michalec
Jul 1, 1997·Thrombosis Research·N H WallénA Bröijersén
Dec 9, 1997·Cell·G S Salvesen, V M Dixit
Jan 23, 1999·Cell Death and Differentiation·N J WaterhouseD J Watters
Mar 20, 1999·Transplantation Proceedings·P LeprinceJ Copeland
Apr 13, 1999·The Journal of Allergy and Clinical Immunology·J J Cohen
Aug 10, 1999·Experimental Cell Research·A K HammillR H Scheuermann
Aug 10, 1999·Experimental Cell Research·E TzimaJ H Walker
Dec 2, 2000·Transfusion·J LiD J Kuter
Jan 11, 2001·Thrombosis Research·P RadziwonH K Breddin
Mar 7, 2002·Analytical Biochemistry·A M WeerheimR Nieuwland
Jul 20, 2002·Cytokine·Pierre Francois PiguetChen Da Kan
Jul 30, 2002·European Journal of Haematology·Pierre F Piguet, Christian Vesin
Dec 14, 2002·Thrombosis Research·Zoë CohenPaul F McDonagh
Mar 29, 2003·Transfusion·Peter L PerrottaEdward L Snyder
May 3, 2003·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Valery Leytin, John Freedman
Jun 26, 2003·Transfusion·A M BertinoD J Kuter
Jun 9, 1962·Nature·G V BORN
Sep 24, 2004·Experimental Neurology·Maryla KrajewskaStan Krajewski
Dec 16, 2004·The Journal of Biological Chemistry·Silvia RiondinoFabio M Pulcinelli
Oct 30, 2009·British Journal of Haematology·Anna ShcherbinaEileen Remold-O'Donnell
Apr 9, 2016·Oxidative Medicine and Cellular Longevity·Jianhai ZhangXiaoming Deng
Citations
Aug 19, 2014·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Yunlong ZhuangChuanfu Zhu
Nov 30, 2013·Cell Death & Disease·I Abd-ElrahmanD Ben-Yehuda
Aug 5, 2005·Oncogene·Sophie LaunayCarmen Garrido
Apr 25, 2008·Life Sciences·José J LópezJuan A Rosado
May 5, 2005·Journal of Thrombosis and Haemostasis : JTH·Y S Ahn
May 20, 2008·Journal of Thrombosis and Haemostasis : JTH·J ShiG E Gilbert
Aug 27, 2010·Transfusion Medicine·P CooksonR Cardigan
Jun 21, 2014·Blood·Jinlin JiangEleftherios T Papoutsakis
Aug 1, 2014·Blood·Michael Keegan DelaneyXiaoping Du
Feb 2, 2012·Blood·Michael J WhiteBenjamin T Kile
May 23, 2018·Blood·Irina PleinesEmma C Josefsson
Apr 12, 2014·Blood Research·Reza ShiriMohammad Reza Tabatabaei
Jan 5, 2019·Transfusion·Ehteramolsadat HosseiniMasood Haghshenas
Jun 15, 2007·Journal of Thrombosis and Haemostasis : JTH·J J LopezJ A Pariente
May 13, 2008·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Zoë CohenLeslie Ritter
Feb 11, 2012·Transfusion·Samantha ReidDenese C Marks
Jul 18, 2017·Journal of Acupuncture and Meridian Studies·Azadeh Meshkini, Masoumeh Tahmasbi
Feb 7, 2018·Thrombosis Research·Mehran GhasemzadehParvin Zadkhak