Caspase-mediated cleavage of the centrosomal proteins during apoptosis

Cell Death & Disease
Mi Young Seo, Kunsoo Rhee

Abstract

The centrosome is the major microtubule-organizing center and plays important roles in intracellular transport, cellular morphology, and motility. In mitotic cells, centrosomes function as spindle poles to pull a set of chromosomes into daughter cells. In quiescent cells, primary cilia are originated from the centrosomes. Given its involvement in various cellular processes, it is little surprising that the organelle would also participate in apoptotic events. However, it remains elusive how the centrosome changes in structure and organization during apoptosis. Apoptosis, a programmed cell death, is required for homeostatic tissue maintenance, embryonic development, stress responses, etc. Activation of caspases generates a cascade of apoptotic pathways, explaining much of what happens during apoptosis. Here, we report the proteolytic cleavage of selected centrosomal proteins in apoptotic cells. SAS-6, a cartwheel component of centrioles, was specifically cleaved at the border of the coiled-coil domain and the disordered C-terminus. Pericentrin, a scaffold of pericentriolar material, was also cleaved during apoptosis. These cleavages were efficiently blocked by the caspase inhibitors. We propose that the caspase-dependent proteol...Continue Reading

References

Dec 1, 1996·The Journal of Cell Biology·L RaoE White
Aug 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pasan FernandoLynn A Megeney
Sep 6, 2002·Oncogene·David V HansenAdam G Eldridge
May 18, 2004·Molecular Biology of the Cell·Wendy C ZimmermanStephen J Doxsey
Sep 18, 2004·Carcinogenesis·Robert Gerl, David L Vaux
Dec 2, 2004·Developmental Cell·Alexander DammermannKaren Oegema
Apr 14, 2005·Journal of Cellular Physiology·Anka G Ehrhardt, Greenfield Sluder
Sep 1, 2005·Journal of Cell Science·Jon D LanePhilip G Woodman
Oct 26, 2005·Nature Cell Biology·Robert HabedanckErich A Nigg
May 26, 2006·Journal of Cell Science·David K MossJon D Lane
Jan 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·José A Sánchez-AlcázarPlácido Navas
Feb 3, 2007·Cell Death and Differentiation·A U Lüthi, S J Martin
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Aug 8, 2007·Developmental Cell·Julia Kleylein-SohnErich A Nigg
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Rebecca C TaylorSeamus J Martin
Dec 18, 2007·Current Biology : CB·Yuki NakazawaMasafumi Hirono
Dec 21, 2007·Molecular Biology of the Cell·Christine DidierNabila Jabrane-Ferrat
Oct 30, 2008·Journal of Cell Science·Thomas V A MurrayHoward O Fearnhead
Dec 17, 2008·Current Biology : CB·Inês Cunha-FerreiraMónica Bettencourt-Dias
Jan 13, 2011·Journal of Cell Science·Kyeongmi Kim, Kunsoo Rhee
Jan 29, 2011·Science·Mark van BreugelBenoît Zuber
Feb 1, 2011·Cell·Daiju KitagawaMichel O Steinmetz
Dec 21, 2011·The Journal of Cell Biology·Kwanwoo Lee, Kunsoo Rhee
May 1, 2012·Current Biology : CB·Kazuhiko MatsuoMikiko Takahashi
Aug 18, 2012·Asia-Pacific Journal of Clinical Oncology·Na Guo, Zhilan Peng
Dec 25, 2012·Molecular & Cellular Proteomics : MCP·Emily D CrawfordJames A Wells
Sep 18, 2015·Methods in Cell Biology·Renata Basto, Karen Oegema

❮ Previous
Next ❯

Citations

Oct 23, 2019·Biological Chemistry·Shiyu ChenMatthew Bogyo
May 31, 2019·Cellular and Molecular Life Sciences : CMLS·Paolo Armando Gagliardi, Luca Primo
Oct 30, 2020·International Journal of Nanomedicine·Dorota K FlakJanka Held-Feindt
May 30, 2021·Biochemical and Biophysical Research Communications·Conor T RonayneVenkatram R Mereddy
Nov 1, 2021·Journal of Molecular Cell Biology·Yongai XiongChunfu Zheng

❮ Previous
Next ❯

Methods Mentioned

BETA
transfections

Software Mentioned

Photoshop
ImagePro

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Related Papers

Biology of Reproduction
Gaurishankar ManandharPeter Sutovsky
Cellular & Molecular Biology Letters
Indrajit ChowdhuryGanapathy K Bhat
© 2022 Meta ULC. All rights reserved