Caspase proteases mediate apoptosis induced by anticancer agent preactivated MC540 in human tumor cell lines

Cancer Letters
S PervaizM V Clément

Abstract

The molecular events involved in tumor cell death induced by novel photoproducts of merocyanine 540 (pMC540) are poorly understood. Using HL60 leukemia and M14 melanoma cell lines we investigated the role of the apoptotic pathway in pMC540-mediated cell death. Tumor cells exposed to pMC540 showed cell size shrinkage and an increase in the sub-diploid DNA content. A loss of membrane phospholipid asymmetry associated with apoptosis was induced by pMC540 in both tumor cell lines as evidenced by the externalization of phosphatidylserine. A dose-dependent increase in caspase-3 protease activity suppressed by the tetrapeptide inhibitor DEVD-CHO was observed in both cell lines. Western blot analysis of poly (ADP-ribose) polymerase, a caspase substrate, showed the classical cleavage pattern (116 to 89 kDa) associated with apoptosis in pMC540-treated cell lysates. Furthermore, caspase inhibition blocked the externalization of membrane PS, indicating that the loss of membrane phospholipid asymmetry is a downstream event of caspase activation. These findings demonstrate that tumor cell death induced by pMC540 is mediated by caspase proteases.

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Citations

May 11, 2002·Journal of Cellular and Molecular Medicine·Robert JesselHorst A Diehl
Jun 3, 2006·Photochemistry and Photobiology·Veronika SackováJán Kleban
Mar 19, 2013·Journal of Photochemistry and Photobiology. B, Biology·Piyali MitraSamita Basu
Sep 2, 2006·Biochemical and Biophysical Research Communications·Patrycja Nowak-SliwinskaKrystyna Urbanska
Oct 20, 1999·FEBS Letters·S PervaizM V Clément
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Dec 6, 2017·Biotechnology & Genetic Engineering Reviews·Goutam Banerjee, Arun Kumar Ray
Aug 29, 2020·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Dongtao WangZiwei Wang
Oct 11, 2001·Oncogene·S PervaizM V Clément

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