Caspase redundancy and release of mitochondrial apoptotic factors characterize interdigital apoptosis

Apoptosis : an International Journal on Programmed Cell Death
V Zuzarte-LuisJ M Hurle

Abstract

Here we show a detailed analysis of cellular and molecular events during in vivo apoptotic cell death in the INZs (interdigital necrotic zones) of the embryonic limb. As the apoptotic mechanisms proceed, the transcriptionally active chromatin and nuclear traffic of RNAs are disrupted, cytoskeletal components are disorganized and the adhesive properties of cells are compromised as Paxillin, a clue member of the focal adhesion complex, decreases in early apoptotic cells. Activation of effector caspases 3 and 7 follow nuclear degradation. In addition, active caspase2 is localized in the nuclei and cytoplasm of early apoptotic cells suggesting a major role in physiological conditions supported by its down-regulation in tissue survival experiments. However in caspase 2 siRNA assays we observed translocation of caspase 3 to the nuclei suggesting functional redundancy. We also observed release of cytochrome c and AIF from the mitochondria, and interestingly AIF becomes intranuclear in a caspase independent manner.

References

Jun 1, 1996·The Journal of Cell Biology·M D JacobsenM C Raff
Jan 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N YasuharaY Tsujimoto
Feb 11, 1998·Experimental Cell Research·E Salas-VidalL Covarrubias
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M JürgensmeierJ C Reed
Jun 6, 1998·Genes & Development·L BergeronJ Yuan
Dec 1, 1999·Cell Death and Differentiation·T S ZhengR A Flavell
Apr 17, 2001·Experimental Cell Research·P D GregoryW Hörz
Jun 26, 2001·Cell Death and Differentiation·Y ByunV L Cryns
Jul 27, 2001·The Journal of Cell Biology·E Arteaga-SolisF Ramirez
Feb 5, 2002·The Journal of Biological Chemistry·Kee-Oh ChayJ Frederic Mushinski
Feb 8, 2002·The Journal of Biological Chemistry·Yin GuoEmad S Alnemri
Jun 13, 2002·The European Journal of Neuroscience·Eva RubioEnrique J de la Rosa
Jul 31, 2002·The Journal of Cell Biology·Sean P CreganRuth S Slack
Mar 26, 2003·Cell Death and Differentiation·U FischerK Schulze-Osthoff
Mar 26, 2003·Cell Death and Differentiation·C M Troy, M L Shelanski
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Steven K HanksJan Brábek
Dec 12, 2003·Development·Damali N Martin, Eric H Baehrecke
Apr 8, 2004·Developmental Cell·Beth Levine, Daniel J Klionsky
Apr 13, 2004·Oncogene·Maria Eugenia GuicciardiGregory J Gores
May 4, 2004·Trends in Pharmacological Sciences·Suk Jin HongValina L Dawson
Jun 3, 2004·The Journal of Biological Chemistry·Klaus W WagnerQuinn L Deveraux

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Citations

Dec 31, 2009·Apoptosis : an International Journal on Programmed Cell Death·Juan A Montero, Juan M Hurlé
Jul 9, 2010·Apoptosis : an International Journal on Programmed Cell Death·J A MonteroJ M Hurle
Nov 22, 2008·Cell Death and Differentiation·G KrumschnabelA Villunger
Mar 23, 2011·Stem Cells and Development·Ricardo Luiz de Azevedo-PereiraRosalia Mendez-Otero
Feb 23, 2011·Development, Growth & Differentiation·Rocío Hernández-Martínez, Luis Covarrubias
Nov 6, 2009·Annual Review of Genetics·Barbara Conradt
Dec 7, 2007·BMC Developmental Biology·Susan AminAbigail S Tucker
Apr 25, 2009·Current Opinion in Ophthalmology·Norman B Javitt, Jonathan C Javitt
Jan 31, 2008·Congenital Anomalies·Philip E Mirkes
Sep 9, 2011·IUBMB Life·Jesús Chimal-MonroyAlejandro Farrera-Hernández
Sep 22, 2006·Developmental Biology·Vanessa Zuzarte-LuisJuan M Hurle
Nov 2, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Eva JanečkováEva Matalová
Aug 17, 2020·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Juan A MonteroJuan M Hurle
May 23, 2020·Cells·Vasanthy Vigneswara, Zubair Ahmed

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