Caspase selective reagents for diagnosing apoptotic mechanisms.

Cell Death and Differentiation
Marcin PorebaGuy S Salvesen

Abstract

Apical caspases initiate and effector caspases execute apoptosis. Reagents that can distinguish between caspases, particularly apical caspases-8, 9, and 10 are scarce and generally nonspecific. Based upon a previously described large-scale screen of peptide-based caspase substrates termed HyCoSuL, we sought to develop reagents to distinguish between apical caspases in order to reveal their function in apoptotic cell death paradigms. To this end, we selected tetrapeptide-based sequences that deliver optimal substrate selectivity and converted them to inhibitors equipped with a detectable tag (activity-based probes-ABPs). We demonstrate a strong relationship between substrate kinetics and ABP kinetics. To evaluate the utility of selective substrates and ABPs, we examined distinct apoptosis pathways in Jurkat T lymphocyte and MDA-MB-231 breast cancer lines triggered to undergo cell death via extrinsic or intrinsic apoptosis. We report the first highly selective substrate appropriate for quantitation of caspase-8 activity during apoptosis. Converting substrates to ABPs promoted loss-of-activity and selectivity, thus we could not define a single ABP capable of detecting individual apical caspases in complex mixtures. To overcome thi...Continue Reading

References

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·T Fernandes-AlnemriE S Alnemri
Mar 21, 1997·The Journal of Biological Chemistry·Q ZhouG S Salvesen
Apr 11, 1997·The Journal of Biological Chemistry·R V TalanianW W Wong
Oct 27, 1997·The Journal of Biological Chemistry·C ScaffidiM E Peter
Oct 9, 1998·The Journal of Biological Chemistry·H R StennickeG S Salvesen
Nov 26, 1998·The Journal of Biological Chemistry·M Garcia-CalvoN A Thornberry
Apr 10, 1999·Methods : a Companion to Methods in Enzymology·H R Stennicke, G S Salvesen
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y LiuB F Cravatt
Mar 23, 2000·Cardiovascular Research·A Saraste, K Pulkki
Aug 23, 2001·Genome Biology·A M VerhagenD L Vaux
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·J WangM J Lenardo
Jun 18, 2002·The Biochemical Journal·Ciara A RyanGuy S Salvesen
Jul 18, 2002·Nature Reviews. Drug Discovery·John C Reed
Dec 12, 2002·Chemical Reviews·Jean-Bernard Denault, Guy S Salvesen
Mar 12, 2003·Toxicology Letters·Marion MacFarlane
Apr 23, 2003·Cancer Letters·Young-Ju JangHyung-Il Kim
Nov 25, 2003·Oncogene·Shulin Wang, Wafik S El-Deiry
Dec 31, 2003·Methods : a Companion to Methods in Enzymology·Dieter BrömmeBoris Turk
Mar 28, 2006·Nature Chemical Biology·Stephan A SieberBenjamin F Cravatt
May 9, 2006·Developmental Cell·Zachary T Schafer, Sally Kornbluth
Sep 2, 2006·Cell Death and Differentiation·B A Callus, D L Vaux
Oct 21, 2006·Cell Death and Differentiation·M LamkanfiP Vandenabeele
Apr 18, 2007·The Biochemical Journal·Jean-Bernard DenaultGuy S Salvesen
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Rebecca C TaylorSeamus J Martin
Nov 4, 2008·Biochemical and Biophysical Research Communications·Natasha Ann Pereira, Zhiwei Song
May 29, 2009·The Journal of Biological Chemistry·Cristina Pop, Guy S Salvesen
Jun 6, 2009·The EMBO Journal·Srinivas MalladiShawn B Bratton
Jul 15, 2009·Nature Medicine·Laura E EdgingtonMatthew Bogyo
Feb 18, 2010·Molecular Cell·Jerry E ChipukDouglas R Green
Nov 15, 2011·Cell Death and Differentiation·L Bouchier-Hayes, D R Green
Jan 17, 2012·Apoptosis : an International Journal on Programmed Cell Death·Karine Sá FerreiraChristoph Borner
Jun 22, 2013·Cold Spring Harbor Perspectives in Biology·Marcin PorebaMarcin Drag
Jul 10, 2013·Chembiochem : a European Journal of Chemical Biology·Chris J VickersDennis W Wolan
Aug 21, 2013·Trends in Cell Biology·Kaleigh Fernald, Manabu Kurokawa

❮ Previous
Next ❯

Citations

Oct 9, 2019·Chemistry, an Asian Journal·Izabela MaluchMarcin Drag
Oct 23, 2019·Biological Chemistry·Shiyu ChenMatthew Bogyo
Jun 13, 2020·Journal of Leukocyte Biology·Pontus Orning, Egil Lien
May 18, 2020·Cell Death and Differentiation·Valentina C Sladky, Andreas Villunger
Apr 13, 2019·Cell Death and Differentiation·Marcin PorebaMarcin Drag
Apr 30, 2019·Organic & Biomolecular Chemistry·Filipe ElvasLeonie Wyffels
Oct 6, 2020·Journal of Receptor and Signal Transduction Research·Jianguo BinYunjun Xu
Feb 9, 2021·Frontiers in Cell and Developmental Biology·Qian LiWeijun Fu
Apr 4, 2021·International Journal of Molecular Sciences·Matej VizovisekSimone Schuerle
Apr 28, 2021·Journal of Medicinal Chemistry·Hong-Rae KimEuna Yoo
Jun 5, 2021·Journal of the American Chemical Society·Scott LovellEdward W Tate
May 18, 2021·Frontiers in Chemistry·Paulina Kasperkiewicz
Sep 2, 2020·Journal of the American Chemical Society·Marcin PorebaGuy S Salvesen

❮ Previous
Next ❯

Methods Mentioned

BETA
pull down
caspase

Software Mentioned

GraphPad Prism
HyCoSuL
Image Studio Lite

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Actin-binding Proteins

Actin-binding proteins are a component of the actin cytoskeleton that play essential roles in cellular functions such as regulation of actin polymerization, maintenance of cell polarity, gene expression regulation, cell motility and many more functions. Discover the latest research on actin-binding proteins here.