Cassette baculovirus vectors for the production of chimeric, humanized, or human antibodies in insect cells

European Journal of Immunology
M A PoulM P Lefranc

Abstract

Plasmid cassette-transfer vectors pBHuC chi and pBHuC gamma l have been designed which enable the construction of recombinant baculoviruses directing the co-expression of complete immunoglobulin in insect cells. We describe the application of these vectors for the expression of a human/mouse chimeric monoclonal antibody of potential immunosuppressive clinical value derived from a mouse anti-human CD29 monoclonal antibody (Mu-K20). The chimeric K20 light and heavy chains produced in sf9 insect cells were correctly processed and assembled into a normal immunoglobulin which is secreted into the culture medium of infected cells. The chimeric mAb Ch-K20-sf9 reproduces in vitro the functional properties of the parental mouse K20, including affinity and inhibition of lymphocyte proliferation. These results demonstrate that the baculovirus/insect cell expression system is suitable for the expression of fully active monoclonal antibodies of therapeutic value. Our generic cassette approach makes this system a very flexible and convenient one for the rapid production of either chimeric, humanized or human mAb with heavy and light chains of any isotype.

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Citations

Apr 12, 2012·Virologica Sinica·Shu-fen LiFei Deng
Dec 1, 1995·Immunotechnology : an International Journal of Immunological Engineering·M A PoulM P Lefranc
Jul 21, 2004·Pathologie-biologie·C Desgranges
Jan 1, 1997·Journal of Virological Methods·H ChaabihiA Berne
Oct 1, 1996·Current Opinion in Biotechnology·I Jones, Y Morikawa
Oct 15, 2009·Protein Engineering, Design & Selection : PEDS·Xin ShenGuan-Xin Shen
Feb 28, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Josée GolayMartine Duonor-Cérutti
Nov 16, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qiang LiHiroshi Shimizu
Oct 28, 2006·The Journal of Biological Chemistry·Rémy RobertGisèle Clofent-Sanchez
Jan 24, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Samuel TroadecThierry Chardès

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