Castor bean isocitrate lyase lacking the putative peroxisomal targeting signal 1 ARM is imported into plant peroxisomes both in vitro and in vivo

Plant Physiology
X GaoA Baker

Abstract

To understand and manipulate plant peroxisomal protein targeting, it is important to establish the universality or otherwise of targeting signals. Contradictory results have been published concerning the nature and location of the glyoxysomal/peroxisomal targeting signal of isocitrate lyase (ICL). L.J. Olsen, W.F. Ettinger, B. Damsz, K. Matsudaira, A. Webb, and J.J. Harada ([1993] Plant Cell 5: 941-952) concluded that the last 5 amino acids (AKSRM) of Brassica napus ICL were sufficient and the last 37 amino acids were necessary for targeting to Arabidopsis leaf peroxisomes. In contrast, R. Behari and A. Baker ([1993]) J Biol Chem 268: 7315-7322) could find no requirement for the almost identical carboxy-terminal sequence AKARM for import of Ricinus communis ICL into isolated sunflower cotyledon glyoxysomes. To resolve this discrepancy, the import characteristics of a mutant R. communis ICL lacking the last 19 amino acids of the carboxy terminus was studied. ICL delta 19 was able to be imported by isolated sunflower glyoxysomes and by tobacco leaf peroxisomes when expressed transgenically. These results demonstrate that the in vitro import system faithfully reflects targeting in vivo, and that the source of the organelles (Arabi...Continue Reading

References

Nov 1, 1992·Free Radical Biology & Medicine·L A del RíoF J Corpas
May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Nov 1, 1969·The Journal of Cell Biology·S E Frederick, E H Newcomb
Jun 1, 1995·Molecular Biology of the Cell·P A WaltonS Subramani
Nov 17, 1995·Cell·R A Rachubinski, S Subramani
Dec 1, 1994·The Journal of Cell Biology·J A McNew, J M Goodman
Apr 1, 1993·Plant Molecular Biology·R Preisig-Müller, H Kindl
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·C GietlM Veenhuis
Jan 1, 1993·Annual Review of Cell Biology·S Subramani
Jun 1, 1993·Plant Molecular Biology·I OnyeochaA Baker
Feb 1, 1993·The Journal of Cell Biology·F KraglerA Hartig

❮ Previous
Next ❯

Citations

Sep 23, 1998·The Plant Journal : for Cell and Molecular Biology·M R PoolA Baker
May 15, 2003·Molecular Membrane Biology·John A ParkesAlison Baker
Sep 29, 2006·Biochimica Et Biophysica Acta·Ida J van der Klei, Marten Veenhuis
Nov 15, 2015·The Journal of Biological Chemistry·Moninder S BhogalAlison Baker
Jan 18, 2005·Plant & Cell Physiology·Katharina NowakRobert Hänsch
Jun 19, 1998·Biochimica Et Biophysica Acta·T N Popova, M A Pinheiro de Carvalho

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.