Catabolism of D-glucose by Pseudomonas putida U occurs via extracellular transformation into D-gluconic acid and induction of a specific gluconate transport system.

Microbiology
Carmen SchleissnerJosé M Luengo

Abstract

Pseudomonas putida U does not degrade D-glucose through the glycolytic pathway but requires (i) its oxidation to D-gluconic acid by a peripherally located constitutive glucose dehydrogenase (insensitive to osmotic shock), (ii) accumulation of D-gluconic acid in the extracellular medium, and (iii) the induction of a specific energy-dependent transport system responsible for the uptake of D-gluconic acid. This uptake system showed maximal rates of transport at 30 degrees C in 50 mM potassium phosphate buffer, pH 7.0. Under these conditions the K(m) calculated for D-gluconic acid was 6.7 microM. Furthermore, a different transport system, specific for the uptake of glucose, was also identified. It is active and shows maximal uptake rates at 35 degrees C in 50 mM potassium phosphate buffer, pH 6.0, with a K(m) value of 8.3 microM.

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Citations

Jun 24, 2010·Applied Microbiology and Biotechnology·Aditi D BuchG Naresh Kumar
Feb 9, 2013·Applied Microbiology and Biotechnology·Paramasivan PonrajParamasamy Gunasekaran
Oct 24, 2002·FEMS Microbiology Letters·Lothar PetruschkaHeidrun Herrmann
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Feb 28, 2001·The EMBO Journal·I Cases, V de Lorenzo
Apr 21, 2009·Applied and Environmental Microbiology·Patrice de WerraMonika Maurhofer
Mar 7, 2006·Applied and Environmental Microbiology·Aditya BasuPrashant S Phale
Feb 25, 2009·Prikladnaia biokhimiia i mikrobiologiia·M GhoshA K Tripathi
Jan 11, 2013·Environmental Microbiology·Max ChavarríaVíctor de Lorenzo
Nov 5, 2002·FEMS Microbiology Reviews·Jean-Charles Portais, Anne-Marie Delort
Jun 1, 2006·FEMS Microbiology Letters·Aditya Basu, Prashant S Phale
Oct 23, 2013·Organic Letters·Guo-Chao XuJian-He Xu
May 22, 2021·Biochimica Et Biophysica Acta. Biomembranes·Eugenia M RapoportNicolai V Bovin

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