PMID: 3989549May 1, 1985

Catabolism of exogenous and endogenous sphingomyelin and phosphatidylcholine by homogenates and subcellular fractions of cultured neuroblastoma cells. Effects of anesthetics

Journal of Neurochemistry
M J MooibroekM W Spence

Abstract

Cultured murine neuroblastoma cells contain a neutral, Mg2+-stimulated sphingomyelinase and an alkaline phosphatidylcholine-hydrolyzing activity that are enriched in the plasma membrane fraction. The reaction products of sphingomyelin catabolism are phosphocholine and ceramide and those of phosphatidylcholine, glycerophosphocholine and fatty acid. These reactions were studied with endogenous as well as exogenous liposomal substrates. With both exogenous and endogenous substrates, the sphingomyelinase activity was stimulated two- to threefold by Mg2+ and a further three- to fourfold by volatile anesthetic agents. Stimulation was concentration-dependent and corresponded to anesthetic potency: methoxyflurane greater than halothane greater than enflurane. Greater than 80% of the plasma membrane sphingomyelin was hydrolyzed within 2 h in the presence of Mg2+ and anesthetic. In contrast, the activity with exogenous and endogenous phosphatidylcholine was unaffected by Mg2+ or Ca2+ and was markedly inhibited (50-80%) by anesthetic agents. The degree of inhibition was concentration-dependent and corresponded to anesthetic potency. The quantitative importance of choline-containing lipids in cell membranes, the relatively exclusive locali...Continue Reading

References

May 1, 1990·Biochimica Et Biophysica Acta·A H Merrill, D D Jones
Sep 1, 1996·Journal of Lipid Mediators and Cell Signalling·G TettamantiL Riboni
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Citations

Aug 1, 1976·Journal of Neurochemistry·J A Rooke, G R Webster
Jan 1, 1976·Advances in Experimental Medicine and Biology·L A HorrocksJ Y Chin
Jan 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·H Woelk, G Porcellati
Feb 1, 1972·Journal of Neurochemistry·M F Cooper, G R Webster
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Jun 4, 1968·Biochimica Et Biophysica Acta·S Gatt
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Jan 1, 1982·Perspectives in Biology and Medicine·F S Labella
Sep 16, 1980·Biochemical and Biophysical Research Communications·K Y Hostetler, L B Hall

Related Concepts

Establishment and Maintenance of Localization
Choline
Halothane
Central Neuroblastoma
Sphingomyelin Catabolic Process
Ceramides
Membrane Fraction
Murine
Neurons
Enflurane

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