Catabolism of native and oxidized low density lipoproteins: in vivo insights from small animal positron emission tomography studies

Amino Acids
Jens PietzschJ van den Hoff

Abstract

The human organism is exposed to numerous processes that generate reactive oxygen species (ROS). ROS may directly or indirectly cause oxidative modification and damage of proteins. Protein oxidation is regarded as a crucial event in the pathogenesis of various diseases ranging from rheumatoid arthritis to Alzheimer's disease and atherosclerosis. As a representative example, oxidation of low density lipoprotein (LDL) is regarded as a crucial event in atherogenesis. Data concerning the role of circulating oxidized LDL (oxLDL) in the development and outcome of diseases are scarce. One reason for this is the shortage of methods for direct assessment of the metabolic fate of circulating oxLDL in vivo. We present an improved methodology based on the radiolabelling of apoB-100 of native LDL (nLDL) and oxLDL, respectively, with the positron emitter fluorine-18 ((18)F) by conjugation with N-succinimidyl-4-[(18)F]fluorobenzoate ([(18)F]SFB). Radiolabelling of both nLDL and oxLDL using [(18)F]SFB causes neither additional oxidative structural modifications of LDL lipids and proteins nor alteration of their biological activity and functionality, respectively, in vitro. The method was further evaluated with respect to the radiopharmacologic...Continue Reading

References

Jan 2, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·J ShepherdH G Morgan
Apr 1, 1992·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·G Vaidyanathan, M R Zalutsky
Jan 1, 1989·Genetic Epidemiology·N J Cox, R S Spielman
Oct 1, 1989·Applied and Environmental Microbiology·R H OltmannsF Lingens
Dec 17, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·S Orrenius
Jan 1, 1988·Atherosclerosis·C L MalmendierC Delcroix
Apr 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G ZhengR T McCluskey
Jul 1, 1993·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·D Schlondorff
Jul 1, 1993·Journal of Biochemical and Biophysical Methods·J RossL P Wennogle
Jun 1, 1996·Current Opinion in Lipidology·P HughD M Foster
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·M P RamprasadD Steinberg
Dec 1, 1996·Biopharmaceutics & Drug Disposition·R J BauerA H Kung
Apr 1, 1997·IEEE Transactions on Medical Imaging·M DefriseD F Newport
Jun 2, 1998·Drug Metabolism Reviews·E R Stadtman, B S Berlett
Aug 1, 1998·The Journal of Biological Chemistry·S Y ChoiA D Cooper
Dec 16, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·T BjörnhedenO Wiklund
Jan 14, 1999·The New England Journal of Medicine·R Ross
Jun 25, 1999·Biochimica Et Biophysica Acta·Y I Miller, N Shaklai
Aug 12, 1999·Free Radical Biology & Medicine·L N GrinbergZ Hrkal
Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·B S Dhaliwal, U P Steinbrecher
Oct 21, 1999·The Journal of Pharmacy and Pharmacology·M Laznicek, A Laznickova
Aug 11, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·V TerpstraT J van Berkel
May 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·A J SchwabK S Pang
Aug 22, 2001·IEEE Transactions on Medical Imaging·X LiuD Townsend

❮ Previous
Next ❯

Citations

Sep 6, 2005·Amino Acids·R Bergmann, J Pietzsch
Dec 6, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Tanize do Espírito Santo FaulinDulcineia Saes Parra Abdalla
Dec 7, 2013·Theranostics·Hakan OrbayWeibo Cai
Oct 19, 2006·Nuclear Medicine and Biology·Christina HultschThomas Henle
Apr 18, 2018·Nuclear Medicine and Molecular Imaging·Atushi NakanoHidehiro Iida
Feb 26, 2016·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ganesan VaidyanathanMichael R Zalutsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alzheimer's Disease: Neuroimaging

Neuroimaging can help identify pathological hallmarks of Alzheimer's disease (AD). Here is the latest research on neuroimaging modalities, including magnetic resonance imaging and positron emission tomography, in AD.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.