PMID: 9440505Jan 24, 1998Paper

Catabolite gene activator protein mutations affecting activity of the araBAD promoter

Journal of Bacteriology
X Zhang, R F Schleif

Abstract

We have studied catabolite gene activator protein (CAP) activation at the araBAD promoter, pBAD, in the absence of DNA looping. We ruled out the two most plausible indirect activation mechanisms: CAP-induced folding of upstream DNA back upon RNA polymerase, and CAP-induced stabilization of AraC binding to DNA. Therefore, a direct CAP-RNA polymerase interaction seemed likely. We sought and found CAP mutants defective in transcription activation at pBAD that retained normal DNA binding affinity. Some mutations altered residues in the interval from positions 150 to 164 that includes CAP activating region 1 (AR1), which has been shown to contact RNA polymerase at a number of promoters. Unexpectedly, additional mutations were found that altered residues in the region between positions 46 and 68 and at position 133. This includes the region known as activating region 3 (AR3). Mutations from both groups also affect the araFGH and rhaBAD promoters.

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Citations

Aug 26, 2000·Journal of Bacteriology·A Dhiman, R Schleif
Nov 5, 2005·Applied and Environmental Microbiology·Alison Buchan, L Nicholas Ornston
Aug 16, 2011·Journal of Bacteriology·Jennifer Seedorff, Robert Schleif
Jul 16, 2020·Cells·Oleg V BylinoYulii V Shidlovskii
Jun 8, 2010·BMC Systems Biology·Carla J DavidsonMichael G Surette
Aug 8, 1998·Journal of Bacteriology·B SaviolaR F Schleif
Mar 12, 1999·Journal of Bacteriology·L D Wonderling, G V Stauffer
Jun 20, 1998·Journal of Molecular Biology·B SaviolaR F Schleif
Apr 5, 2001·Journal of Molecular Biology·M Wu, R Schleif
Nov 5, 1999·Journal of Molecular Biology·S Busby, R H Ebright
Dec 5, 2000·Trends in Genetics : TIG·R Schleif
May 10, 2001·Biochimica Et Biophysica Acta·J G Harman

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