Catabolite repression in the hyperthermophilic bacterium Thermotoga neapolitana is independent of cAMP.

Microbiology
Madeline Vargas, K M Noll

Abstract

Thermotoga neapolitana is a hyperthermophilic bacterium whose phylogenetic lineage includes the most primitive of the bacterial heterotrophs. It is not known whether Thermotoga exhibits preferences for growth substrates or regulates the synthesis of degradative enzymes. We have found that T. neapolitana exhibits diauxic growth in medium containing 300 microM glucose and 1 mM lactose. We measured the activity of beta-galactosidase and beta-glucosidase in extracts prepared from cells grown on defined media and found that cells grown on 0.5% lactose, galactose or cellobiose contained beta-galactosidase specific activities of 1.19, 1.78 and 1.34 U (mg protein)-1, respectively. Cells grown on 0.5% glucose, maltose, fructose, sucrose, xylose, ribose or starch had no measurable beta-galactosidase activity. beta-Glucosidase activity was found only in cells grown on cellobiose. Cells grown on the combination of 0.5% lactose or galactose and 0.05% glucose had no detectable beta-galactosidase activity, whereas up to 0.5% glucose did not prevent expression of beta-galactosidase or beta-glucosidase activity in cells induced with 0.5% cellobiose. These activities are catalysed by separate enzymes as determined by resolution of their activiti...Continue Reading

References

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Citations

Oct 21, 2010·Biotechnology Letters·Niels T EriksenNiels Iversen
Feb 13, 2001·FEMS Microbiology Letters·T N NguyenK M Noll
Aug 21, 2013·Applied Microbiology and Biotechnology·Sudhanshu S Pawar, Ed W J van Niel
Jul 24, 2008·Metabolic Engineering·Chung-Jung ChouRobert M Kelly
Oct 27, 2006·FEMS Microbiology Reviews·Shannon B ConnersRobert M Kelly
Dec 12, 2002·The Journal of Biological Chemistry·Swapnil R ChhabraRobert M Kelly
Jan 17, 2012·Applied and Environmental Microbiology·Andrew D FrockRobert M Kelly
Mar 1, 1997·Applied and Environmental Microbiology·M Y GalperinA H Romano

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