Catabolite repression of the toluene degradation pathway in Pseudomonas putida harboring pWW0 under various conditions of nutrient limitation in chemostat culture.

Applied and Environmental Microbiology
W A DuetzJ G van Andel

Abstract

In earlier studies, the pathway of toluene and m- and p-xylene degradation (TOL pathway) in Pseudomonas putida (pWW0) was found to be subject to catabolite repression when the strain was grown at the maximal rate on glucose or succinate in the presence of an inducer. This report describes catabolite repression of the TOL pathway by succinate in chemostat cultures run at a low dilution rate (D = 0.05 h-1) under different conditions of inorganic-nutrient limitation. The activity of benzylalcohol dehydrogenase (BADH) in cell extracts was used as a measure of the expression of the TOL upper pathway. When cells were grown in the presence of 10 to 15 mM succinate under conditions of phosphate or sulfate limitation, the BADH activity in response to the nonmetabolizable inducer o-xylene was less than 2% of that of cells grown under conditions of succinate limitation. Less repression was found under conditions of ammonium or oxygen limitation (2 to 10% and 20 to 35%, respectively, of the BADH levels under succinate limitation). The BADH expression levels determined under the different growth conditions appeared to correlate well with the mRNA transcript levels from the upper pathway promoter (Pu), which indicates that repression was due...Continue Reading

References

Dec 1, 1990·Journal of Bacteriology·N Hugouvieux-Cotte-PattatS Harayama
Jan 1, 1990·Advances in Microbial Physiology·S J Assinder, P A Williams
May 1, 1986·Applied and Environmental Microbiology·T A Shaler, G M Klecka
Jun 11, 1971·European Journal of Biochemistry·J M Sala-Trepat, W C Evans
Jun 11, 1982·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·W Harder, L Dijkhuizen
Feb 1, 1995·Applied and Environmental Microbiology·K O'ConnorA D Dobson
Sep 1, 1993·Molecular Microbiology·S Marqués, J L Ramos
Aug 1, 1994·Bulletin of Environmental Contamination and Toxicology·S A Mills, W T Frankenberger
Apr 1, 1994·The Journal of Applied Bacteriology·M A Ramadan
May 1, 1954·The Biochemical Journal·I S LONGMUIR
Mar 1, 1986·Applied and Environmental Microbiology·D L LewisR E Hodson
Feb 1, 1995·Environmental Science & Technology·I M CozzarelliM J Baedecker

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Citations

May 26, 2001·Applied and Environmental Microbiology·W A DuetzB Witholt
Feb 3, 2005·Nature Reviews. Microbiology·Ildefonso Cases, Víctor de Lorenzo
Aug 9, 2005·Applied and Environmental Microbiology·Isabel Aranda-OlmedoSilvia Marqués
Aug 19, 2007·Journal of Bacteriology·Annette Arndt, Bernhard J Eikmanns
Jan 1, 2009·Microbial Biotechnology·Trygve BrautasetSvein Valla
Jul 14, 2000·Journal of Bacteriology·M BuckJ D Gralla
Mar 1, 2012·Applied Microbiology and Biotechnology·Kaoru Ikuma, Claudia K Gunsch
Feb 16, 2006·Environmental Microbiology·Leonid KurbatovLothar Petruschka
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Mar 28, 2008·Environmental Microbiology·Wei E HuangAndrew S Whiteley
Jan 22, 1998·Applied and Environmental Microbiology·F AmpeN D Lindley
Aug 10, 2000·Metabolic Engineering·H C Winther-LarsenS Valla
Oct 1, 1996·Applied and Environmental Microbiology·K O'ConnorA D Dobson

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