Catabolite responsive elements as a strategy for the control of heterologous gene expression in lactobacilli.

Applied Microbiology and Biotechnology
Susana LangaJosé María Landete

Abstract

Genes involved in the transport and catabolism of carbohydrates are usually controlled through the binding of the catabolite control protein A (CcpA) to the catabolite-responsive elements (cre) of target genes in Gram-positive bacteria. In this work, we show how the elimination of the cre sites in Lactobacillus casei BL23 promoters induced by sorbitol (PgutF), maltose (PmalL), and myo-inositol (PiolT) allowed the induction of gene expression in media supplemented with sorbitol, maltose, and myo-inositol, respectively, even in the presence of glucose. This was studied using plasmids encoding the anaerobic fluorescent protein evoglow-Pp1 as a reporter. In addition, gutF cre site was introduced into a bile inducible promoter (P16090) and into the constitutive promoter of the elongation factor P (PEf-P) of L. casei BL23. The existence of the cre site blocked gene expression in the P16090 inducible promoter in the presence of glucose, while it had no influence on the expression of the PEf-P constitutive one. These results demonstrated that the introduction or elimination of cre sites in inducible promoters allows the control and modification of their heterologous genetic expression, showing how the cre site, the transcriptional regu...Continue Reading

References

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Citations

Jul 3, 2021·ACS Synthetic Biology·Yupeng ZhangGuiyang Shi
Sep 24, 2021·Journal of Industrial Microbiology & Biotechnology·Syed S ShahMargaret L Britz

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Methods Mentioned

BETA
phosphotransferase
PCRs

Software Mentioned

SPSS

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