PMID: 7541472Mar 1, 1995Paper

Catalase and hydrogen peroxide cytotoxicity in cultured cardiac myocytes

Journal of Molecular and Cellular Cardiology
L D Horwitz, J A Leff

Abstract

We examined the role of intracellular catalase activity in modulating hydrogen peroxide (H2O2)-induced cytotoxicity in cultured chick embryo cardiac myocytes. Injury was quantitated by release of lactate dehydrogenase (LDH). Application of 1.5 mM H2O2 to myocytes caused LDH release beginning at 2 h. Inactivation or inhibition of catalase with aminotriazole or sodium azide increased LDH release but did not cause earlier release. Free catalase which entered or became associated with myocytes, but not catalase bound to agarose beads, which did not enter or become associated with myocytes, was protective. Separate experiments demonstrated that myocyte catalase activity decreased by 27% between 1 and 4 h of H2O2 exposure. Treatment with aprotinin, a protease inhibitor, prevented the H2O2-induced fall in catalase activity at 4 h but treatment with deferoxamine, an iron chelator, had no effect on catalase activity. Thus, with exposure of cardiac myocytes to H2O2, the magnitude of the cytotoxicity is modulated by endogenous or cell associated exogenous catalase. It is proposed that in addition to excessive accumulation of H2O2, a reduction intracellular catalase activity may be required before substantial cell injury occurs during H2O2...Continue Reading

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Citations

Nov 7, 2000·Free Radical Biology & Medicine·I DurotP Athias
Jan 23, 1999·Free Radical Biology & Medicine·L M Winn, P G Wells
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·L D HorwitzM A Horwitz
Dec 1, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shen-Feng MaMitsuru Hashida
Sep 2, 1999·The Journal of Thoracic and Cardiovascular Surgery·M HendrikxU Mees
Mar 23, 1999·The Annals of Thoracic Surgery·C F WippermannH Oelert
Jul 16, 1999·Vascular Medicine·L D Horwitz, E A Rosenthal
Sep 22, 2017·Current Medicinal Chemistry·Hana Jansová, Tomáś Šimůnek
Jul 23, 1999·Annals of the New York Academy of Sciences·J G Kingma
Nov 22, 2000·American Journal of Physiology. Heart and Circulatory Physiology·S W Rabkin, J Y Kong

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