PMID: 9537289Apr 16, 1998Paper

Catalase and oviductal fluid reverse the decreased motility of bovine sperm in culture medium containing specific amino acids

Journal of Andrology
S Lapointe, M A Sirard

Abstract

The motility and velocity of bovine spermatozoa incubated in TCM-199 are reduced in comparison with those incubated in a simpler media made for sperm, such as modified Tyrode (Sp-Talp). Moreover, a previous study showed that oviductal cells conditioned media prevented this decreased motility in TCM-199. Preliminary results lead us to suspect that amino acids in TCM-199 were involved in the reduced survival. Therefore, the current experiment aimed at determining which amino acids were involved and what their mechanism of action involved. Amino acids were added separately in Sp-Talp at the final concentration found in TCM-199. Frozen-thawed bovine spermatozoa were washed twice by centrifugation in Sp-Talp and diluted to 25 x 10(6)/ml in the amino acid media. After 6 hours of incubation at 37 degrees C, sperm motility and velocity were recorded. The percentage of motile sperm was significantly lower in the presence of phenylalanine (6%+/-2, P < 0.05) compared with the control (46%+/-2). Sperm velocity (VAP, microm/ second) was lower in the presence of phenylalanine (50+/-4) and tyrosine (89+/-3) compared with the control (119+/-4, P < 0.05). Increased concentrations of the three aromatic amino acids (0, 0.2, 1, five times TCM-199 ...Continue Reading

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