Catalase down-regulation in cancer cells exposed to arsenic trioxide is involved in their increased sensitivity to a pro-oxidant treatment

Cancer Cell International
Christophe Glorieux, Pedro Buc Calderon

Abstract

Pro-oxidant drugs have been proposed for treating certain cancers but the resistance developed by cancer cells to oxidative stress limits its potential use in clinics. To understand the mechanisms underlying resistance to oxidative stress, we found that the chronic exposure to an H2O2-generating system (ascorbate/menadione, Asc/Men) or catalase overexpression (CAT3 cells) increased the resistance of cancer cells to oxidative stress, likely by increasing the antioxidant status of cancer cells. Modulation of catalase expression was performed by either protein overexpression or protein down-regulation using siRNA against catalase and aminotriazole as pharmacological inhibitor. The former approach was done by transfecting cells with a plasmid construct containing human catalase cDNA (CAT3 cells, derived from MCF-7 breast cancer cell line) or by generating resistant cells through chronic exposure to an oxidant injury (Resox cells). Cell survival was monitored by using the MTT reduction assay and further calculation of IC50values. Protein expression was done by Western blots procedures. The formation of reactive oxygen species was performed by flow cytometry. The transcriptional activity of humancatalasepromoter was assessed by using...Continue Reading

References

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Citations

May 24, 2019·Free Radical Research·David L NewmanStephen L Gregory
Jul 2, 2020·Cancers·Dilip NarayananDennis Özcelik
Apr 3, 2020·Environmental Science and Pollution Research International·Beata Koim-PuchowskaPiotr Kaminski
Dec 8, 2019·Cancer Letters·Malgorzata FirczukRadoslaw Zagozdzon
Nov 19, 2020·Oxidative Medicine and Cellular Longevity·Rumiana BakalovaTatsuya Higashi
Jan 4, 2020·Environmental Pollution·Pablo Sánchez-VirostaTapio Eeva
Mar 18, 2021·Anais Da Academia Brasileira De Ciências·Weibing LanGuangzai Nong
Jun 9, 2020·Redox Biology·José M PalmaFrancisco J Corpas
Jun 16, 2021·Free Radical Biology & Medicine·Marilisa GalassoMaria Teresa Scupoli
Aug 8, 2021·International Journal of Molecular Sciences·Malin Tordis MeyerSrikanth Karnati
Jul 14, 2021·Antioxidants & Redox Signaling·Christophe Glorieux, Pedro Buc Calderon

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Methods Mentioned

BETA
transfection
Assay
PCR
flow cytometry

Software Mentioned

GraphPad Prism
ImageJ

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