Catalase (KatA) plays a role in protection against anaerobic nitric oxide in Pseudomonas aeruginosa

PloS One
Shengchang SuDaniel J Hassett

Abstract

Pseudomonas aeruginosa (PA) is a common bacterial pathogen, responsible for a high incidence of nosocomial and respiratory infections. KatA is the major catalase of PA that detoxifies hydrogen peroxide (H2O2), a reactive oxygen intermediate generated during aerobic respiration. Paradoxically, PA displays elevated KatA activity under anaerobic growth conditions where the substrate of KatA, H2O2, is not produced. The aim of the present study is to elucidate the mechanism underlying this phenomenon and define the role of KatA in PA during anaerobiosis using genetic, biochemical and biophysical approaches. We demonstrated that anaerobic wild-type PAO1 cells yielded higher levels of katA transcription and expression than aerobic cells, whereas a nitrite reductase mutant ΔnirS produced ∼50% the KatA activity of PAO1, suggesting that a basal NO level was required for the increased KatA activity. We also found that transcription of the katA gene was controlled, in part, by the master anaerobic regulator, ANR. A ΔkatA mutant and a mucoid mucA22 ΔkatA bacteria demonstrated increased sensitivity to acidified nitrite (an NO generator) in anaerobic planktonic and biofilm cultures. EPR spectra of anaerobic bacteria showed that levels of dini...Continue Reading

References

Oct 2, 1979·Molecular & General Genetics : MGG·A M Chakrabarty, I C Gunsalus
Mar 1, 1994·The Biochemical Journal·R G Knowles, S Moncada
Aug 15, 1995·European Journal of Biochemistry·G C Brown
Apr 1, 1995·American Journal of Respiratory and Critical Care Medicine·T Z KhanD W Riches
Apr 4, 1995·Biochimica Et Biophysica Acta·H AraiT Kodama
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D W MartinV Deretic
Jul 22, 1997·Proceedings of the National Academy of Sciences of the United States of America·D A Siegele, J C Hu
Oct 6, 1997·The Journal of Biological Chemistry·P R GardnerA L Salzman
Oct 3, 1999·Applied and Environmental Microbiology·J G ElkinsT R McDermott
Mar 7, 2001·Applied and Environmental Microbiology·J R FrederickT R McDermott
Feb 6, 2002·The Journal of Clinical Investigation·Dieter WorlitzschGerd Döring
Feb 22, 2002·Microbial Pathogenesis·Sabine Favre-BontéChristian van Delden
Jul 3, 2002·The EMBO Journal·Hugo Cruz-RamosRobert K Poole
Sep 25, 2002·Biochemistry·Anatoly L Mayburd, Richard J Kassner
Dec 3, 2002·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Emmanuelle Soum, Jean-Claude Drapier
Dec 18, 2002·Journal of Bacteriology·Shery VargheseJames A Imlay
Apr 18, 2003·Journal of Bacteriology·George A O'Toole
Jun 15, 2005·Annual Review of Biochemistry·Deborah C JohnsonMichael K Johnson

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Citations

Oct 2, 2015·Current Opinion in Microbiology·Andrés Vázquez-Torres, Andreas J Bäumler
Aug 27, 2014·Infection and Immunity·Sahar A Alshalchi, Gregory G Anderson
Sep 26, 2019·Journal of Basic Microbiology·Gang ZhouQing-Shan Shi
Sep 13, 2019·Journal of Industrial Microbiology & Biotechnology·Gang ZhouQing-Shan Shi
May 31, 2019·Clinical Microbiology Reviews·Sankalp MalhotraDaniel J Wozniak
Apr 20, 2019·Scientific Reports·Yolanda Elisabet González-FloresEduardo Santero
Feb 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Cameron T McDanielDaniel J Hassett
Jun 30, 2019·Extremophiles : Life Under Extreme Conditions·Esmeralda C Solar VeneroNancy I López
Mar 2, 2021·Acta Crystallographica. Section D, Structural Biology·Mariana G SartorioJavier M González
Apr 4, 2021·International Journal of Molecular Sciences·Irene Jurado-MartínSiobhán McClean
Jun 3, 2021·Antioxidants·Vitaliy B BorisovElena Forte

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Methods Mentioned

BETA
electrophoresis
PCR
X-ray
Light Microscopy
chemical treatment

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