PMID: 9553756Apr 29, 1998Paper

Catalase transfection decreases hydrogen peroxide toxicity in a pancreatic beta cell line

Endocrine Research
P M KralikP N Epstein

Abstract

BetaTC6-F7 cells like normal Beta cells were found to be highly sensitive to hydrogen peroxide and to possess very low levels of catalase. Therefore we tested whether overexpression of catalase could enhance resistance to hydrogen peroxide. Enzyme activity was increased forty fold by transient transfection of a catalase transgene. To assess protection from hydrogen peroxide a cotransfection method using a human growth hormone reporter gene was developed. Human growth hormone secretion was shown to be a suitable marker for insulin secretion since both hormones demonstrated virtually identical glucose dose response curves. Catalase transfection was found to provide significant protection against hydrogen peroxide indicating that low catalase may contribute to the sensitivity of cells to hydrogen peroxide.

References

May 1, 1990·Journal of Biochemistry·S Furuta, H Hayashi
Oct 1, 1988·Analytical Biochemistry·L H Johansson, L A Borg
Feb 1, 1987·The Journal of Clinical Investigation·C F Nathan
Nov 12, 1981·Nature·K GrankvistI B Täljedal
Jan 1, 1994·Methods in Cell Biology·T C BeckerC B Newgard
Jul 5, 1996·Science·R S Sohal, R Weindruch

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adenoma, Islet Cell

Islet Cell Adenoma arises in the islet cells, which are insulin producing cells of the pancreas. These tumors can be either malignant or benign. Discover the latest research on Islet Cell Adenoma here.