Catalogue of epidermal genes: genes expressed in the epidermis during larval molt of the silkworm Bombyx mori.

BMC Genomics
Shun OkamotoHaruhiko Fujiwara

Abstract

The insect cuticle is composed of various proteins and formed during the molt under hormonal regulation, although its precise composition and formation mechanism are largely unknown. The exhaustive catalogue of genes expressed in epidermis at the molt constitutes a massive amount of information from which to draw a complete picture of the molt and cuticle formation in insects. Therefore, we have catalogued a library of full-length cDNAs (designated epM) from epidermal cells during the last larval molt of Bombyx mori. Of the 10,368 sequences in the library, we isolated 6,653 usable expressed sequence tags (ESTs), which were categorized into 1,451 nonredundant gene clusters. Seventy-one clusters were considered to be isoforms or premature forms of other clusters. Therefore, we have identified 1,380 putative genes. Of the 6,653 expressed sequences, 48% were derived from 92 cuticular protein genes (RR-1, 24; RR-2, 17; glycine-rich, 29; other classes, 22). A comparison of epM with another epidermal EST data set, epV3 (feeding stage: fifth instar, day 3), showed marked differences in cuticular protein gene. Various types of cuticular proteins are expressed in epM but virtually only RR-1 proteins were expressed in epV3. Cuticular prot...Continue Reading

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Citations

Mar 17, 2010·BMC Genomics·Jiubo LiangNingjia He
Dec 7, 2013·PloS One·Jothini Odman-NareshHans Merzendorfer
Jan 13, 2015·Insect Biochemistry and Molecular Biology·Neal T DittmerMichael R Kanost
Jan 12, 2010·Insect Biochemistry and Molecular Biology·J P Charles
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May 1, 2015·BMC Genomics·Seanna J McTaggartMike Boots
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Datasets Mentioned

BETA
DC432783

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
epM
SignalP3
InterProScan
Blastx

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