Catalpol alleviates adriamycin-induced nephropathy by activating the SIRT1 signalling pathway in vivo and in vitro

British Journal of Pharmacology
Jiangnan ZhangKexin Liu

Abstract

Catalpol, a water-soluble active ingredient isolated from Rehmannia glutinosa, exhibits multiple pharmacological activities. However, the mechanism(s) underlying protection against renal injury by catalpol remains unknown. Adriamycin-induced kidney injury models associated with podocyte damage were employed to investigate the nephroprotective effects of catalpol. In vivo, TUNEL and haematoxylin-eosin staining was used to evaluate the effect of catalpol on kidney injury in mice. In vitro, effects of catalpol on podocyte damage induced by adriamycin was determined by elisa kit, flow cytometry, Hoechst 33342, and TUNEL staining. The mechanism was investigated by siRNA, EX527, and docking simulations. In vivo, catalpol treatment significantly improved adriamycin-induced kidney pathological changes and decreased the number of apoptotic cells. In vitro, catalpol markedly decreased the intracellular accumulation of adriamycin and reduced the calcium ion level in podocytes and then attenuated apoptosis. Importantly, the regulatory effects of catalpol on sirtuin 1 (SIRT1), multidrug resistance-associated protein 2 (MRP2), and the TRPC6 channel were mostly abolished after incubation with SIRT1 siRNA or the SIRT1-specific inhibitor EX527....Continue Reading

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Citations

Mar 27, 2020·Frontiers in Pharmacology·Qiongzhen ChenFanghua Gong
Oct 30, 2020·Neuropsychiatric Disease and Treatment·Jing Wang, Xingmao Wu
Jan 7, 2021·International Journal of Molecular Sciences·Christopher L MooreAlexei G Basnakian
Feb 7, 2021·Basic & Clinical Pharmacology & Toxicology·Ali A Shati, Attalla F El-Kott
Apr 16, 2021·Frontiers in Pharmacology·Mingming ZhaoYu Zhang
Jul 31, 2021·Frontiers in Pharmacology·Lei HeXuehui Wang
Nov 9, 2021·Nucleic Acids Research·Tingting FuFeng Zhu

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